Translationally controlled tumor protein interacts with the third cytoplasmic domain of Na,K-ATPase alpha subunit and inhibits the pump activity in HeLa cells

The Journal of Biological Chemistry
Jaehoon JungKyunglim Lee

Abstract

Translationally controlled tumor protein (TCTP) is a growth-related protein under transcriptional as well as translational control. We screened a rat skeletal muscle cDNA library using yeast two-hybrid system and found that TCTP interacts with the third large cytoplasmic domain of alpha1 as well as alpha2 isoforms of Na,K-ATPase, believed involved in the regulation of Na,K-ATPase activity. Interaction between TCTP and Na,K-ATPase was confirmed by coimmunoprecipitation in yeast and mammalian cells. We also showed, using (86)Rb(+) uptake assay, that overexpression of TCTP inhibited Na,K-ATPase activity in HeLa cells. Northern and Western blotting studies of HeLa cells transiently transfected with GFP-tagged TCTP showed that overexpression of TCTP did not change mRNA and protein levels of Na,K-ATPase. Recombinant TCTP protein purified from an Escherichia coli expression system inhibited purified HeLa cell plasma membrane Na,K-ATPase in a dose-dependent manner. Using deletion analysis, we also found that the C-terminal 102-172-amino-acid region of rat TCTP that contains the TCTP homology region 2 is essential for its association with, and inhibition of, Na,K-ATPase.

References

Jan 1, 1979·Annual Review of Physiology·K J Ullrich
Nov 1, 1990·Journal of Cellular Physiology·J J Kort, G Koch
Oct 25, 1989·Nucleic Acids Research·B GrossH Bielka
Jul 1, 1988·The Journal of Membrane Biology·P L Jørgensen, J P Andersen
Jun 14, 1982·Biochimica Et Biophysica Acta·R B Lingham, A K Sen
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·P DevarajanJ S Morrow
Dec 15, 1995·The Journal of Biological Chemistry·C JordanD Drenckhahn
Dec 1, 1996·Japanese Journal of Pharmacology·Y MurataA Baba
Oct 29, 1997·Archives of Biochemistry and Biophysics·M V Coppi, G Guidotti
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·G A YudowskiA M Bertorello
Aug 16, 2000·American Journal of Physiology. Cell Physiology·A G Therien, R Blostein
Jan 5, 2001·The Biochemical Journal·K LeeG Guidotti
Oct 13, 2001·The Journal of Biological Chemistry·F LiK Fujise
May 25, 2002·European Journal of Biochemistry·Zijian Xie, Amir Askari
Aug 9, 2002·Molecular and Cellular Biology·Frederic R Yarm

❮ Previous
Next ❯

Citations

Aug 19, 2007·The British Journal of Nutrition·Yajun XuYong Li
Jun 25, 2005·Molecular Biology of the Cell·Zhaokan YuanZijian Xie
Nov 8, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mordecai P BlausteinW Gil Wier
Jun 8, 2012·Biochemistry Research International·Michiyo Nagano-Ito, Shinichi Ichikawa
Feb 3, 2007·Biological & Pharmaceutical Bulletin·Jie QiuMei Cheng
Jun 15, 2012·Cellular and Molecular Life Sciences : CMLS·Linda ReinhardPoul Nissen
Apr 10, 2015·International Journal of Molecular Sciences·Jeehye MaengKyunglim Lee
Mar 20, 2013·The Journal of Physiology·Orlaith B KellyStephen J Keely
Sep 2, 2005·Molecular Microbiology·Toru TakahashiKeietsu Abe
Jul 12, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Venugopal Thayanithy, Thayanithy Venugopal
Jul 1, 2007·Cell Adhesion & Migration·Kwang-Wook Choi, Ya-Chieh Hsu
Nov 18, 2010·FEBS Letters·Seung Bae RhoSang-Yoon Park
Jun 12, 2014·International Journal of Molecular Sciences·Jeehye MaengKyunglim Lee
Nov 10, 2011·Toxins·Olga Meiri ChaimSilvio Sanches Veiga
Jun 20, 2020·PLoS Genetics·Sung-Ryeong LeeKwang-Wook Choi
Apr 14, 2017·PloS One·Elaiya Raja SubramanianSudhakar Sivasubramaniam
Jun 6, 2018·International Journal of Molecular Sciences·Jiwon JungKyunglim Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.