Translocating myonuclei have distinct leading and lagging edges that require kinesin and dynein

Development
Eric S FolkerMary K Baylies

Abstract

Nuclei are precisely positioned within all cells, and mispositioned nuclei are a hallmark of many muscle diseases. Myonuclear positioning is dependent on Kinesin and Dynein, but interactions between these motor proteins and their mechanisms of action are unclear. We find that in developing Drosophila muscles, Dynein and Kinesin work together to move nuclei in a single direction by two separate mechanisms that are spatially segregated. First, the two motors work together in a sequential pathway that acts from the cell cortex at the muscle poles. This mechanism requires Kinesin-dependent localization of Dynein to cell cortex near the muscle pole. From this location Dynein can pull microtubule minus-ends and the attached myonuclei toward the muscle pole. Second, the motors exert forces directly on individual nuclei independently of the cortical pathway. However, the activities of the two motors on the nucleus are polarized relative to the direction of myonuclear translocation: Kinesin acts at the leading edge of the nucleus, whereas Dynein acts at the lagging edge of the nucleus. Consistent with the activities of Kinesin and Dynein being polarized on the nucleus, nuclei rarely change direction, and those that do, reorient to maint...Continue Reading

References

May 23, 1998·The Journal of Cell Biology·J G GindhartL S Goldstein
Oct 26, 1999·The Journal of Biological Chemistry·K M BrendzaW M Saxton
Jun 19, 2003·The Journal of Physiology·J C BruusgaardK Gundersen
Mar 30, 2004·Molecular Biology of the Cell·Viacheslav MalikovVladimir Rodionov
Dec 13, 2005·Molecular Biology of the Cell·Anne StraubeGero Steinberg
Nov 15, 2008·Human Molecular Genetics·Megan J PuckelwartzElizabeth M McNally
Jun 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kai LeiMin Han
Jul 17, 2009·Development·Marina MeyerzonDaniel A Starr
Dec 1, 2009·Journal of Molecular and Cellular Cardiology·Megan J PuckelwartzElizabeth M McNally
Dec 17, 2009·Developmental Biology·Heidi N FridolfssonDaniel A Starr
Feb 26, 2010·Neuromuscular Disorders : NMD·Norma Beatriz Romero
Oct 6, 2010·The Journal of Cell Biology·Heidi N Fridolfsson, Daniel A Starr
May 17, 2011·PLoS Computational Biology·Robert P EricksonClare C Yu
May 25, 2012·Journal of Cell Science·Meredith H Wilson, Erika L F Holzbaur
Aug 29, 2012·The Journal of Cell Biology·Hadas Elhanany-TamirTalila Volk

❮ Previous
Next ❯

Citations

Mar 12, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Victoria K SchulmanMary K Baylies
May 27, 2015·The Journal of Cell Biology·Shuoshuo WangTalila Volk
Dec 19, 2014·PLoS Genetics·Victoria K SchulmanMary K Baylies
Feb 27, 2017·Cytoskeleton·Ryan Hickey, Andrew E Pelling
Apr 14, 2017·Molecular Biology of the Cell·Alison K SpencerJennifer A Zallen
Jun 12, 2018·PLoS Computational Biology·Angelika ManhartAlex Mogilner
May 18, 2016·Journal of Cell Science·Courtney R Bone, Daniel A Starr
Dec 5, 2018·Development·Zhonghui FeiAshley E E Bruce
Jun 7, 2020·Cells·Robert BeckerFelix B Engel
Mar 1, 2018·Journal of Cell Science·Alexander L AuldEric S Folker
Apr 14, 2018·The Journal of Cell Biology·Shuoshuo WangTalila Volk
Sep 9, 2018·The Journal of Cell Biology·Tanmay P LeleGregg G Gundersen
Feb 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tomas RojasChenglai Fu
Jun 17, 2016·Molecular Biology of the Cell·Alexander L Auld, Eric S Folker
Jan 11, 2019·The Journal of Cell Biology·Jonathan N RosenMary K Baylies
Apr 18, 2018·Nature Reviews. Molecular Cell Biology·Samara L Reck-PetersonAndrew P Carter
Nov 18, 2018·Frontiers in Physiology·Margherita Perillo, Eric S Folker
Sep 4, 2020·Journal of Cachexia, Sarcopenia and Muscle·Kevin A MurachCory M Dungan
Feb 6, 2020·Trends in Cell Biology·Mafalda Azevedo, Mary K Baylies
Dec 18, 2014·Development·Meredith H Wilson, Erika L F Holzbaur
Oct 14, 2021·Seminars in Cell & Developmental Biology·Ojas Deshpande, Ivo A Telley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.