Translocation of a channel-forming antimicrobial peptide, magainin 2, across lipid bilayers by forming a pore

Biochemistry
K MatsuzakiK Miyajima

Abstract

A channel-forming antimicrobial peptide, magainin 2, has been shown to translocate across phospholipid bilayers by forming a pore comprising multimeric peptides. The translocation was demonstrated by four sets of experiments by use of resonance energy transfer from tryptophan introduced into the peptide to a dansyl chromophore incorporated into the lipid membrane. The translocation was coupled to pore formation, as detected by the dye efflux from the lipid vesicles; about 30% of the total peptide molecules translocated into the inner leaflets over 10 min, while 80% of the dye molecules leaked out at a lipid to peptide ratio of 57. This novel model can explain the problems debated so far, i.e., the peptide forms an ion channel whereas the magainin helix essentially lies parallel to the membrane surface. Channel (pore) formation in the vesicles is a transient process observable mainly during the early stage of the peptide membrane interactions.

References

Aug 3, 1992·European Journal of Pharmacology·R A CrucianiE F Stanley
Apr 1, 1992·Biophysical Chemistry·G Schwarz, C H Robert
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R A CrucianiO Colamonici
Jan 1, 1991·Progress in Biophysics and Molecular Biology·M S Sansom
Mar 18, 1991·Biochimica Et Biophysica Acta·K MatsuzakiK Miyajima
Apr 9, 1991·FEBS Letters·C E DempseyI D Campbell
May 7, 1990·Biochimica Et Biophysica Acta·C E Dempsey
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H V WesterhoffM Zasloff
May 19, 1989·Biochimica Et Biophysica Acta·K MatsuzakiK Miyajima
Feb 13, 1989·Biochimica Et Biophysica Acta·G Schwarz, G Beschiaschvili
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M ZasloffH C Chen
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A I de Kroon, H M McConnell
Feb 23, 1994·Biochimica Et Biophysica Acta·S J LudtkeH W Huang
Dec 1, 1993·Protein Science : a Publication of the Protein Society·B BechingerS J Opella
Nov 1, 1991·Biophysical Journal·H W Huang, Y Wu

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Citations

Aug 10, 2000·Biopolymers·A TossiA Giangaspero
Mar 1, 1996·Protein Science : a Publication of the Protein Society·K S TaylorR M Garavito
Dec 3, 2005·European Biophysics Journal : EBJ·Alice GlättliWilfred F van Gunsteren
Feb 3, 2007·European Biophysics Journal : EBJ·August AnderssonLena Mäler
Jan 29, 2011·European Biophysics Journal : EBJ·Davor JuretićAlessandro Tossi
Feb 22, 2011·European Biophysics Journal : EBJ·Katrin Splith, Ines Neundorf
Dec 1, 1996·Solid State Nuclear Magnetic Resonance·B BechingerS J Opella
May 3, 2005·International Journal of Food Microbiology·R Bauer, L M T Dicks
Mar 17, 2004·Peptides·Jon-Paul S Powers, Robert E W Hancock
Feb 25, 2003·Chemistry and Physics of Lipids·Luís M S LouraManuel Prieto
May 12, 2001·FEMS Microbiology Reviews·O McAuliffeC Hill
Nov 7, 1998·Biochimica Et Biophysica Acta·K Matsuzaki
Oct 25, 2013·Journal of the American Chemical Society·Sterling A WheatenPaulo F Almeida
Oct 3, 2009·Journal of the American Chemical Society·Santi Esteban-MartínSiewert J Marrink
Jul 29, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·J F Liang, S C Kim
Mar 15, 2006·Nature Reviews. Drug Discovery·Eefjan Breukink, Ben de Kruijff
Oct 31, 2001·European Journal of Biochemistry·A GiangasperoA Tossi
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Robert Bucki, Paul A Janmey
May 28, 2009·Antimicrobial Agents and Chemotherapy·Fuminori YoneyamaKenji Sonomoto
Feb 19, 2002·Antimicrobial Agents and Chemotherapy·Aleksander PatrzykatRobert E W Hancock
Mar 29, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J M GirardetG Humbert
Apr 29, 2008·Annual Review of Phytopathology·Jose F MarcosBelén López-García
Nov 21, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Satoe Kobayashi
Dec 12, 2012·PloS One·Shee-Mei LokScott F Michael
Dec 17, 2011·International Journal of Molecular Sciences·Sammy Al-BennaLars Steinstraesser
Feb 9, 2012·Journal of Visualized Experiments : JoVE·Sara A SpinellaDonald E Elmore
Jul 6, 2000·Bioscience, Biotechnology, and Biochemistry·S MachidaY Yu

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