PMID: 7544680Jan 1, 1994Paper

Translocation of an RNA duplex on a ribozyme

Nature Structural Biology
S A Strobel, T R Cech

Abstract

RNA cleavage by the Tetrahymena ribozyme requires recognition of the reaction-site helix by the catalytic apparatus. This binding can occur in several registers, each of which results in reaction at a different nucleotide in the helix. We now identify commensurate sets of 2'-hydroxyl interactions on both strands of the reaction-site helix that account for its translocation into alternative binding registers. These results indicate that the ribozyme has a relatively rigid substrate-binding pocket into which the helix can bind in different alignments. A similar mechanism of reaction site recognition is proposed to occur during intron circularization and ribozyme polymerase activity. Translocation of the reaction site duplex provides an example of structural heterogeneity in packing of helices during the tertiary folding of RNA.

References

Dec 20, 1991·Journal of Molecular Biology·M YarusE Christian
Jun 15, 1989·Nature·J A Doudna, J W Szostak
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A DoudnaJ W Szostak
Nov 23, 1989·Nature·F MichelJ W Szostak

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Citations

Dec 10, 1998·Biopolymers·S A Strobel
Jun 1, 1995·Current Opinion in Structural Biology·A M Pyle, J B Green
Jul 1, 1997·Trends in Biochemical Sciences·S A Strobel, J A Doudna
Jan 1, 1994·Nature Structural Biology·F Michel, E Westhof
May 27, 2008·Nature Structural & Molecular Biology·Feng Qiao, Thomas R Cech
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·G J NarlikarD Herschlag
Jan 1, 1997·Annual Review of Biochemistry·G J Narlikar, D Herschlag
Aug 13, 2013·Biophysical Chemistry·Antonella Paladino, Ronen Zangi
Apr 3, 2002·Chemistry & Biology·Kathleen E McGinness, Gerald F Joyce
Mar 13, 1999·Chemistry & Biology·S A Strobel, L Ortoleva-Donnelly
Sep 6, 2000·Journal of Molecular Biology·J K Strauss-Soukup, S A Strobel
Feb 12, 2013·Journal of Chemical Theory and Computation·Antonella Paladino, Ronen Zangi

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