PMID: 9188810May 22, 1997Paper

Translocation of annexin XI to neutrophil subcellular organelles

Biochimica Et Biophysica Acta
C SjölinC Dahlgren

Abstract

In an earlier study, annexin XI was found to be present in the cytosol of neutrophil granulocytes (Blood (1996) 87, 4817). The protein was isolated by calcium-dependent translocation to specific granules and was found to be a 42-kDa truncated form of annexin XI. Using human autoantibodies directed against annexin XI we have now reinvestigated the ability of full size annexin XI to translocate to different neutrophil organelles isolated by subcellular fractionation. The autoantisera used recognised a protein of 55-kDa in neutrophil cytosol and comparison with a whole cell lysate indicated that the larger portion of the cellular content of this protein is localised to the cytosol. Azurophil granules, specific granules and secretory vesicles/plasma membrane were isolated by subcellular fractionation on Percoll gradients, mixed respectively with neutrophil cytosol and the calcium concentration was raised. Immunoblotting showed that annexin XI translocated to specific granules and secretory vesicles/plasma membrane at 100 micromol/l calcium. When raising the concentration of calcium to 1 mmol/l, annexin XI translocated to the azurophil granules as well. Periphagosomal translocation of annexin XI occurred during phagocytosis of yeast...Continue Reading

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Citations

Nov 14, 2003·Microbes and Infection·Mikkel Faurschou, Niels Borregaard
Jan 5, 2000·Journal of Immunological Methods·L KjeldsenN Borregaard
Feb 11, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jiasheng WangFrederick T Greenaway
Apr 25, 2006·British Journal of Pharmacology·Simon YonaRoderick J Flower
Mar 22, 2000·Electrophoresis·M Boussac, J Garin
May 16, 2019·Journal of Leukocyte Biology·Johannes WestmanMichelle E Maxson
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Apr 20, 2010·Journal of Leukocyte Biology·Paula Nunes, Nicolas Demaurex
Apr 30, 2020·Biomolecules·Peder A G LillebostadPetri Kursula
Aug 26, 1998·Biochimica Et Biophysica Acta·A Szewczyk, S Pikuła

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