PMID: 4895218Mar 1, 1969Paper

Translocation of mRNA codons. II. Properties of an anti-translocase antibody

Proceedings of the National Academy of Sciences of the United States of America
P LEDERD Roufa

Abstract

A purified preparation of translocase, one of several enzymes required for protein biosynthesis, has been used to prepare a specific anti-translocase antibody. This antibody provides an extremely useful tool not only for detecting the enzyme independent of its activity, but for inhibiting the translocase-mediated reaction and, thus, protein biosynthesis. Though the antibody very rapidly inhibits elongation of the peptide chain, it fails to effect initiation, binding, or peptide bond formation, which strongly suggests that translocase is not a direct participant in these reactions. It also arrests translation of the defined tricodon AUG(U(6)) at the second codon, permitting formation only of the dipeptide, fMet-Phe, rather than the tripeptide, fMetPhe-(Phe)(2), which is formed in the presence of control serum. We have shown previously that the third codon becomes available only in the presence of translocase, thereby defining the site of action of the antibody. The antibody also has been used to demonstrate that translocase is antigenically distinct from the other proteins required for initiation and protein synthesis in E. coli.

References

Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·Y Nishizuka, F Lipmann
Sep 26, 1966·Archives of Biochemistry and Biophysics·Y Nishizuka, F Lipmann
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J Lucas-Lenard, F Lipmann
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·P LederM M Nau
Jan 1, 1966·Annual Review of Biochemistry·R Schweet, R Heintz
Oct 20, 1966·Biochemical and Biophysical Research Communications·P Leder, H Bursztyn
Jun 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J M Ravel
Jun 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M SalasS Ochoa
Jun 10, 1968·Biochemical and Biophysical Research Communications·R W Erbe, P Leder

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Citations

Jan 1, 1972·Molecular & General Genetics : MGG·Y Aharonowitz, E Z Ron
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·L SkogersonP Leder
Apr 24, 1970·Biochemical and Biophysical Research Communications·D J RoufaP Leder
Sep 30, 1970·Biochemical and Biophysical Research Communications·M Yukioka, S Morisawa

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