Translocation of phospholipids and dithionite permeability in liquid-ordered and liquid-disordered membranes.

Biophysical Journal
Maria João MorenoWinchil L C Vaz

Abstract

We present a detailed study of the translocation rate of two headgroup-labeled phospholipid derivatives, one with two acyl chains, NBD-DMPE, and the other with a single acyl chain, NBD-lysoMPE, in lipid bilayer membranes in the liquid-disordered state (POPC) and in the liquid-ordered states (POPC/cholesterol (Chol), molar ratio 1:1, and sphingomyelin (SpM)/Chol, molar ratio 6:4). The study was performed as a function of temperature and the thermodynamic parameters of the translocation process have been obtained. The most important findings are 1), the translocation of NBD-DMPE is significantly faster than the translocation of NBD-lysoMPE for all bilayer compositions and temperatures tested; and 2), for both phospholipid derivatives, the translocation in POPC bilayers is approximately 1 order of magnitude faster than in POPC/Chol (1:1) bilayers and approximately 2-3 orders of magnitude faster than in SpM/Chol (6:4) bilayers. The permeability of the lipid bilayers to dithionite has also been measured. In liquid disordered membranes, the permeability rate constant obtained is comparable to the translocation rate constant of NBD-DMPE. However, in liquid-ordered bilayers, the permeability of dithionite is significantly faster then t...Continue Reading

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Citations

May 10, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Yasaman Dayani, Noah Malmstadt
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Marzuk M KamalJonathon Howard
Mar 8, 2016·Biochimica Et Biophysica Acta·Mariusz Kepczynski, Tomasz Róg
Aug 5, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Filipe M Coreta-GomesMaria João Moreno
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Nov 4, 2010·Biophysical Journal·Gustavo FuertesJesús Salgado
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Jun 17, 2020·The Journal of Physical Chemistry. B·Samiron Kumar SahaMasahito Yamazaki
Oct 1, 2021·International Journal of Pharmaceutics·Hugo A L FilipeFilipe E Antunes

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