PMID: 2510160Oct 1, 1989Paper

Translocation of synapsin I in response to depolarization of isolated nerve terminals

Proceedings of the National Academy of Sciences of the United States of America
T S SihraP Greengard

Abstract

Depolarization of isolated nerve terminals (synaptosomes) has been shown to stimulate neurotransmitter release and to increase the phosphorylation state of a number of proteins, including synapsin I, in a Ca2+-dependent manner. Synapsin I, a prominent nerve terminal phosphoprotein, interacts with the cytoplasmic surface of small synaptic vesicles and with cytoskeletal elements in a phosphorylation-dependent manner. In the present study we have found that depolarization of synaptosomes resulted in a rapid (2-5 sec) translocation of synapsin I from the particulate to the cytosolic (soluble) fraction. This translocation of synapsin I correlated with its phosphorylation state and was dependent on the presence of Ca2+ in the incubation medium. The stoichiometry of phosphorylation of soluble synapsin I was considerably higher than that of synapsin I in the particulate fraction, under both basal and depolarizing conditions. These data are consistent with the hypothesis that, in situ, the phosphorylation of synapsin I promotes its translocation from synaptic vesicles/cytoskeleton to the cytosol. This phosphorylation/translocation may be instrumental in regulating the release of neurotransmitter.

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Citations

Jan 1, 1992·Molecular Neurobiology·P Paggi, T C Petrucci
May 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·R Chapell, P Rosenberg
Jan 1, 1995·Neuroscience·W Volknandt
Aug 1, 1991·Brain Research Bulletin·A F Sikorski, S R Goodman
Jan 1, 1995·Brain Research Bulletin·S R GoodmanM L Bloom
Oct 1, 1995·Current Opinion in Neurobiology·P De CamilliP S McPherson
Jun 1, 2000·Progress in Neurobiology·A M Thomson
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D W Kahn, J M Besterman
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·F BenfenatiP Greengard
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·N SahyounE G Lapetina
Nov 1, 1991·The Journal of Cell Biology·M MatteoliP De Camilli
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S HilfikerP Greengard
Feb 1, 1992·Journal of Neurochemistry·R A NicholsP Greengard
Dec 26, 2009·Cellular and Molecular Life Sciences : CMLS·Eugenio F FornasieroFlavia Valtorta
Jul 30, 2011·Seminars in Cell & Developmental Biology·Oleg ShupliakovArndt Pechstein
Jul 30, 2011·Seminars in Cell & Developmental Biology·Flavia ValtortaEugenio F Fornasiero
Jun 7, 2005·Journal of Neurochemistry·Lydie Lane-GuermonprezFabio Benfenati
Jan 19, 2012·The European Journal of Neuroscience·Maya M DavisAngus C Nairn
Jul 1, 1993·Journal of Neurochemistry·R Jahn, T C Südhof
Apr 25, 2007·Journal of Neuroscience Research·E EvergrenO Shupliakov

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