Translocation of the pAntp peptide and its amphipathic analogue AP-2AL

Biochemistry
G DrinRobert Brasseur

Abstract

The pAntp peptide, corresponding to the third helix of the homeodomain of the Antennapedia protein, enters by a receptor-independent process into eukaryotic cells. The interaction between the pAntp peptide and the phospholipid matrix of the plasma membrane seems to be the first step involved in the translocation mechanism. However, the mechanism by which the peptide translocates through the cell membrane is still not well established. We have investigated the translocation ability of pAntp through a protein-free phospholipid membrane in comparison with a more amphipathic analogue. We show by fluorescence spectroscopy, circular dichroism, NMR spectroscopy, and molecular modeling that pAntp is not sufficiently helically amphipathic to cross a phospholipid membrane of a model system. Due to its primary sequence related to its DNA binding ability in the Antennapedia homeodomain-DNA complex, the pAntp peptide does not belong to the amphipathic alpha-helical peptide family whose members are able to translocate by pore formation.

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Jul 26, 1996·The Journal of Biological Chemistry·D DerossiA Prochiantz
Aug 1, 1997·Biophysical Journal·K MatsuzakiK Miyajima
Nov 7, 1998·Biochimica Et Biophysica Acta·R M Epand
May 14, 1999·Journal of Peptide Science : an Official Publication of the European Peptide Society·A SchellerM Bienert
Apr 1, 1995·Journal of Biomolecular NMR·G LippensJ M Wieruszeski
Apr 1, 1986·Journal of Computational Chemistry·Scott J WeinerDavid A Case

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Citations

Jan 31, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Rui ZengMin Han
Mar 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Raphael GorodetskyGerard Marx
Feb 25, 2003·Chemistry and Physics of Lipids·Luís M S LouraManuel Prieto
Feb 5, 2003·Advanced Drug Delivery Reviews·Ching-Hsuan Tung, Ralph Weissleder
Jan 5, 2002·Journal of Molecular Graphics & Modelling·F BasynR Brasseur
Jun 12, 2003·Journal of Molecular Graphics & Modelling·Frederic BasynRobert Brasseur
Oct 16, 2012·Journal of the American Chemical Society·Nathan W SchmidtGerard C L Wong
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Abhijit MishraGerard C L Wong
Jul 16, 2003·The Journal of General Physiology·Paul K KienkerAlan Finkelstein
Mar 11, 2004·European Journal of Biochemistry·Bart ChristiaensMaryvonne Rosseneu
Feb 15, 2007·PloS One·Antonin LamazièreJesus Ayala-Sanmartin
Feb 25, 2014·ACS Nano·Dana Maria CopoloviciÜlo Langel
Oct 16, 2004·Molecular and Cellular Neurosciences·Gunnar P H Dietz, Mathias Bähr
Aug 13, 2013·Organic & Biomolecular Chemistry·Gabriela A EggimannJean-Louis Reymond
Nov 10, 2009·Cellular and Molecular Life Sciences : CMLS·F Said HassaneBernard Lebleu
Jun 27, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Evelyne SchrankKlaus Zangger
Nov 21, 2009·FEBS Letters·Nathan SchmidtGerard C L Wong
Sep 2, 2003·Journal of Pharmaceutical Sciences·Lisa A Kueltzo, C Russell Middaugh
May 15, 2013·FEBS Letters·Chérine Bechara, Sandrine Sagan
Apr 1, 2004·Biopolymers·Jimena Fernández-CarneadoErnest Giralt
Mar 3, 2009·Acta Biomaterialia·Lorenzo AulisaJeffrey D Hartgerink
Mar 3, 2007·Chembiochem : a European Journal of Chemical Biology·Sandro KellerAlfred Blume
Nov 6, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Isabel D AlvesGérard Chassaing
Oct 29, 2005·Chembiochem : a European Journal of Chemical Biology·Rainer FischerRoland Brock
Apr 15, 2005·Biophysical Chemistry·Per E G ThorénBengt Nordén
Dec 3, 2004·Drug Discovery Today·Jamal Temsamani, Pierre Vidal
Jul 30, 2003·Biophysical Journal·Hans Binder, Göran Lindblom
Oct 23, 2012·Biochimica Et Biophysica Acta·Jesper Søborg BahnsenHanne Mørck Nielsen
Jul 21, 2010·Biophysical Journal·Yen SunHuey W Huang
Jul 26, 2005·Biophysical Journal·Elsa Bárány-WalljeMargitta Dathe
May 21, 2010·Biophysical Journal·Chang-Chun LeeHuey W Huang
Oct 9, 2004·Biochimica Et Biophysica Acta·Daniel PerssonBengt Nordén
Mar 15, 2008·Biochimica Et Biophysica Acta·Loïc HugoninAstrid Gräslund

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