Translocation of the retinoblastoma gene product during mitosis

Experimental Cell Research
R C ThomasR Marks

Abstract

This study reports the immunocytochemical localization of the retinoblastoma gene product within synchronized normal human keratinocytes. Data suggest that mitotic spindles function in the transport of the retinoblastoma tumor suppressor gene product during cell division. A diffuse anti-pRB reactivity was detected within the nuclei of G1-phase keratinocytes, although staining was not evident within the nucleoli. During S-phase and G2-phase the anti-pRB reactivity was localized to discrete regions within the nuclear compartment. The anti-pRB reactivity of M-phase cells was localized to the mitotic spindles and microtubule nucleation centers. Immunoprecipitation and Western blotting of the pRB antigen from synchronized keratinocytes showed that the apparent polypeptide molecular weight of pRB increased from 105 kDa during G1-phase to 115 kDa during M-phase. Immunoprecipitation of the pRB antigen from mitotic spindles resulted in the coprecipitation of two polypeptides with apparent polypeptide molecular weights of 115 and 50 kDa. Western blotting of the immunoprecipitates from purified keratinocyte mitotic spindles showed that beta-tubulin was the 50-kDa polypeptide associated with hyperphosphorylated pRB.

Citations

Mar 27, 2003·International Journal of Experimental Pathology·Mauricio SalcedoPatricio Gariglio
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·L C Hsu, R L White
Apr 15, 2009·Biochemical and Biophysical Research Communications·Joseph Ahlander, Giovanni Bosco
Apr 8, 2006·Medical Hypotheses·Ying Chen, Qingzhong Kong
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Jan 23, 2020·Journal of Neurogenetics·Christine N SerwayJ Steven de Belle
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