Translocation of verotoxin-1 across T84 monolayers: mechanism of bacterial toxin penetration of epithelium

The American Journal of Physiology
D J PhilpottP M Sherman

Abstract

Verotoxin-producing Escherichia coli (VTEC) are pathogenic bacteria associated with diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. Verotoxins (VTs) elaborated by these organisms produce cytopathic effects on a restricted number of cell types, including endothelial cells lining the microvasculature of the bowel and the kidney. Because human intestinal epithelial cells lack the globotriaosylceramide receptor for VT binding, it is unclear how the toxin moves across the intestinal mucosa to the systemic circulation. The aims of this study were to determine the effects of VT-1 on intestinal epithelial cell function and to characterize VT-1 translocation across monolayers of T84 cells, an intestinal epithelial cell line. VT-1 at concentrations up to 1 microgram/ml had no effect on the barrier function of T84 monolayers as assessed by measuring transmonolayer electrical resistance (102 +/- 8% of control monolayers). In contrast, both VT-positive and VT-negative VTEC bacterial strains lowered T84 transmonolayer resistance (45 +/- 7 and 38 +/- 6% of controls, respectively). Comparable amounts of toxin moved across monolayers of T84 cells, exhibiting high-resistance values, as monolayers with VTEC-induced decreases in barr...Continue Reading

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Citations

Nov 1, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Irina MalyukovaOlga Kovbasnjuk
Aug 13, 2011·American Journal of Physiology. Cell Physiology·Valeriy LukyanenkoOlga Kovbasnjuk
Mar 10, 2010·Future Microbiology·Vernon L Tesh
Mar 3, 2019·Glycobiology·Hyun-Kyu ChoiHung-Jen Wu
Sep 9, 2016·Frontiers in Cellular and Infection Microbiology·Paul Ugalde-SilvaFernando Navarro-Garcia
May 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·N L JonesP M Sherman
May 30, 2001·Microbes and Infection·E V O'Loughlin, R M Robins-Browne
Dec 23, 2014·Microbiology Spectrum·Angela R Melton-Celsa

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