PMID: 1203749Dec 18, 1975Paper

Transmembrane bone matrix gelatin-induced differentiation of bone

Calcified Tissue Research
H Nogami, M R Urist

Abstract

In response to chemically-defined bone matrix gelatin (BMG) inside a diffusion chamber implanted in a muscle pouch, mesenchymal cells migrate directionally, aggregate and differentiate into new bone, on the outside of the chamber. BMG diffuses through double membranes 275 to 300 mum in thickness. The inner membrane of pore size is 0.025 mum and the outer membrane of pore size is 0.45 mum. The inner membrane is 1/20 the pore size and the combination is twice the thickness of membranes previously reported to transfer osteoinductive activity of living cells. Autoradiographs show 35S-cysteine-labelled BMG produces very high trans-membrane grain counts while 3H-proline labelled BMG produces very low transmembrane grain counts. Electron micrographs demonstrate that gelatin-derived, uranyl-acetate-stained fine granules interspersed with ruthenium red-staining coarse granules, diffuse through the membrane of 0.025 mum pore size from the inside out. Solitary pale-staining collagen fibrils, possibly formed in interstitial fluid by renaturation of BMG are found in the interior of the chamber and in the interior of the outer 0.45 mum but not the inner 0.025 mum pore size membrane. Densely-stained new bone collagen fiber bundles cover the o...Continue Reading

References

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Citations

Feb 1, 1997·Mund-, Kiefer- und Gesichtschirurgie : MKG·N R Kübler
Oct 28, 2003·DNA and Cell Biology·M Bücheler, A Haisch
Sep 2, 2009·Dental Materials Journal·Kenjiro NakanoTatsushi Kawai
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Jan 1, 1992·Bone and Mineral·H C AndersonT Hunt
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Dec 1, 1993·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·N KüblerW Sebald

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