PMID: 6977432Jan 1, 1981Paper

Transmembrane components of taurine flux across frog retinal pigment epithelium

Current Eye Research
T J Ostwald, R H Steinberg

Abstract

In previous work from this laboratory, a net transepithelial flux of the amino acid taurine was measured across the in vitro frog retinal pigment epithelium. This flux was from retina to choroid and could be modulated by small (0.5 mM) changes in K+ concentration, by changes in taurine concentration, and by ouabain. In the present experiments we measured the unidirectional transmembrane fluxes across each of the two cell membranes, the apical membrane (facing the neural retina) and the basal membrane (facing the choroid) of the retinal pigment epithelium. In modified Ringer's solution containing 2mM taurine + 2mM K+, we found that the apparent outward permeability of the basal membrane, corrected for its actual area, was 26 times that of the apical membrane. As expected from the direction of net flux, the inward apical and outward basal fluxes dominated the transmembrane fluxes. When the apical Na+:K+ pump was inhibited, the ratio of the apparent permeability of the basal membrane relative to the apical decreased from 26 to 4.4. The data are consistent with the previous suggestion of Na+:taurine co-transport into the retinal pigment epithelium across the apical membrane. The basal membrane response to ouabain and reduced K+ con...Continue Reading

References

Jan 1, 1975·Annual Review of Physiology·I M Glynn, S J Karlish
Sep 1, 1977·Experimental Eye Research·S S Miller, R H Steinberg
Dec 29, 1978·The Journal of Membrane Biology·S S MillerB Oakley
Dec 29, 1978·The Journal of Membrane Biology·B OakleyR H Steinberg
Aug 1, 1979·The Journal of General Physiology·S S Miller, R H Steinberg
Mar 1, 1976·Journal of Neurochemistry·H T OrrO H Lowry
Jul 1, 1976·Journal of Neurochemistry·A J Kennedy, M J Voaden
Jul 1, 1972·Vision Research·M S Starr, M J Voaden
Aug 24, 1973·Experimental Eye Research·R H Steinberg, S Miller
Jan 1, 1974·The Journal of Membrane Biology·R Naftalin, P F Curran
Oct 1, 1970·Physiological Reviews·S G Schultz, P F Curran
Sep 1, 1980·Experimental Eye Research·T J Ostwald, R H Steinberg

❮ Previous
Next ❯

Citations

Aug 1, 1992·Experimental Eye Research·B G Kennedy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.