Transmembrane electrical and pH gradients across human erythrocytes and human peripheral lymphocytes

Journal of Cellular Physiology
C DeutschD F Wilson

Abstract

Transmembrane electrical and pH gradients have been measured across human erythrocytes and peripheral blood lymphocytes using equilibrium distributions of radioactively labelled lipophilic ions, and of weak acids and weak bases, respectively. The distributions of methylamine, trimethylamine, acetic acid and trimethylacetic acid give calculated transmembrane pH gradients (pHe-pHi) for erythrocytes of between 0.14-0.21 for extracellular pH values of 7.28-7.16. The distributions of trimethylacetic acid. DMO and trimethylamine were determined for lymphocytes, establishing upper and lower limits of the calculated pH gradient over the external pH range of 6.7 to 7.7. Tritiated triphenylmethyl phosphonium ion (TPMP) and 14C-thiocyanate ion (SCN) equilibrium distributions were measured in order to calculate transmembrane electrical potentials, using tetraphenylboron as a catalyst to facilitate TPMP equilibrium. Transmembrane potentials of -7 to -10 mV were calculated from SCN and TPMP, respectively for red cells, and -35 to -52 mV respectively, in the case of lymphocytes. Distributions of TPMP and potassium ions were determined in the presence of valinomycin over a wide range of extracellular potassium concentrations for red cells and ...Continue Reading

References

Aug 1, 1976·Clinical Science and Molecular Medicine·J M BoneA T Lambie
Jan 6, 1977·Biochimica Et Biophysica Acta·G F AzzoneP D Antone
Mar 1, 1977·The Journal of General Physiology·P A KnaufA Rothstein
Feb 1, 1978·The Journal of Experimental Medicine·R P DanieleP C Nowell
Aug 1, 1975·The Journal of Physiology·M Dalmark
Feb 9, 1978·Biochimica Et Biophysica Acta·G F AzzoneM Bragadin
Jan 1, 1978·Biophysical Journal·O S AndersenS McLaughlin
May 6, 1977·The Journal of Membrane Biology·L G Palmer, M M Civan
Aug 1, 1976·Experimental Cell Research·W Negendank, C R Collier
Dec 1, 1976·The Journal of Clinical Investigation·G B Segel, M A Lichtman
Dec 30, 1975·Annals of the New York Academy of Sciences·E HeinzC Pietrzyk
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R P Daniele, S K Holian
Aug 4, 1970·Biochimica Et Biophysica Acta·L E BakeevaV P Skulachev
Dec 1, 1972·Journal of Cellular Physiology·M A LichtmanW A Peck
Jun 1, 1966·Endocrinology·F Maloof, M Soodak
Feb 18, 1974·Annals of the New York Academy of Sciences·F J LombardiH R Kaback
Aug 4, 1970·Biochimica Et Biophysica Acta·L L GriniusM A Vladimirova
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H HirataF M Harold
Sep 1, 1972·Experimental Cell Research·T F McDonaldJ D Ebert
Dec 1, 1972·Journal of Cellular Physiology·H G Sachs, T F McDonald
Mar 1, 1973·The American Journal of Physiology·M B Burg, N Green
May 15, 1973·Experientia·C M Feldherr
Feb 1, 1974·Experimental Cell Research·H G SachsJ D Ebert
Apr 1, 1969·Transactions of the New York Academy of Sciences·C D Cone
Jan 1, 1971·Journal of Theoretical Biology·C D Cone
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
Apr 1, 1968·The Journal of Physiology·U V Lassen, O Sten-Knudsen
Jan 1, 1966·Scandinavian Journal of Clinical and Laboratory Investigation·J Funder, J O Wieth
May 1, 1967·The American Journal of Physiology·P D ZieveJ R Krevans

Citations

Dec 1, 1989·Clinical Biochemistry·N K OzerC A Pasternak
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H KieferH R Kaback
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H M Shapiro, T B Strom
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C DeutschD F Wilson
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M PriceC Deutsch
Aug 1, 1987·The Journal of Cell Biology·K SandvigB van Deurs
Jun 1, 1982·The Journal of Cell Biology·R B MikkelsenD F Wallach
Feb 1, 1984·The Journal of Cell Biology·L TanasugarnD L Taylor
Mar 1, 1996·The Journal of General Physiology·G Panyi, C Deutsch
Mar 1, 1984·The Journal of General Physiology·S GrinsteinA Rothstein
Jun 1, 1996·British Journal of Obstetrics and Gynaecology·L M BoltonW Dunlop
Nov 5, 2008·BMC Immunology·Fernanda Mello de QueirozGuilherme Suarez-Kurtz
Feb 9, 1994·Molecular and Cellular Biochemistry·E J AiyathuraiB S Moonga
Apr 1, 1990·Journal of Cellular Physiology·T I TønnessenS Olsnes
Dec 1, 1983·Cell Calcium·T J Rink, C Deutsch
Mar 1, 1981·Bioscience Reports·C L Bashford
Feb 1, 1980·Journal of Cellular Physiology·R B MikkelsenD F Wallach
Oct 1, 1982·Journal of Cellular Physiology·C Deutsch, M Price
Jul 1, 1981·Journal of Cellular Physiology·A H Bui, J S Wiley
Dec 1, 1982·Journal of Cellular Physiology·W Negendank, C Shaller
Oct 1, 1984·Journal of Cellular Physiology·S GrinsteinE W Gelfand
Nov 1, 1988·Quarterly Reviews of Biophysics·J MatkóS Damjanovich
Dec 1, 1979·FEBS Letters·C Deutsch, U Rafalowska
Jan 1, 1988·Journal of Cellular Biochemistry·K SandvigB van Deurs
Jan 1, 1980·Journal of Receptor Research·R B Mikkelsen, D L Freerksen
Jan 1, 1982·Journal of Immunopharmacology·K L AudusM A Gordon
Aug 1, 1984·Current Eye Research·K A BowmanK Green

Related Concepts

Acetic Acids
Erythrocytes
Hydrogen-Ion Concentration
Lymphoid Cells
Resting Potentials
Methylamines
Potassium
Trityl Compounds

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.