Transmembrane peptides influence the affinity of sterols for phospholipid bilayers.

Biophysical Journal
Joel H NyströmThomas Nyholm

Abstract

Cholesterol is distributed unevenly between different cellular membrane compartments, and the cholesterol content increases from the inner bilayers toward the plasma membrane. It has been suggested that this cholesterol gradient is important in the sorting of transmembrane proteins. Cholesterol has also been to shown play an important role in lateral organization of eukaryotic cell membranes. In this study the aim was to determine how transmembrane proteins influence the lateral distribution of cholesterol in phospholipid bilayers. Insight into this can be obtained by studying how cholesterol interacts with bilayer membranes of different composition in the presence of designed peptides that mimic the transmembrane helices of proteins. For this purpose we developed an assay in which the partitioning of the fluorescent cholesterol analog CTL between LUVs and mbetaCD can be measured. Comparison of how cholesterol and CTL partitioning between mbetaCD and phospholipid bilayers with different composition suggests that CTL sensed changes in bilayer composition similarly as cholesterol. Therefore, the results obtained with CTL can be used to understand cholesterol distribution in lipid bilayers. The effect of WALP23 on CTL partitioning...Continue Reading

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Citations

Apr 19, 2011·European Biophysics Journal : EBJ·Terhi MaulaJ Peter Slotte
Jun 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Thomas K M NyholmJ Peter Slotte
Jan 21, 2016·Biophysical Journal·Tomokazu YasudaJ Peter Slotte
Sep 18, 2012·Biochimica Et Biophysica Acta·Shishir Jaikishan, J Peter Slotte
Jan 26, 2011·Biochimica Et Biophysica Acta·Anders BjörkbomJ Peter Slotte
Apr 26, 2011·Biochimica Et Biophysica Acta·Shishir Jaikishan, J Peter Slotte
Jan 5, 2011·Biochimica Et Biophysica Acta·Christian SergeliusShigeo Katsumura
May 26, 2015·Biochimica Et Biophysica Acta·Daniel Wüstner, Katarzyna Solanko
Jun 4, 2015·Chemistry and Physics of Lipids·Thomas K M Nyholm
Nov 30, 2010·Biophysical Journal·Frédérick J-M de MeyerBerend Smit
Dec 4, 2012·Biophysical Journal·Terhi MaulaJ Peter Slotte
Aug 11, 2016·Biophysical Journal·Oskar EngbergThomas K M Nyholm
Oct 21, 2016·Biophysical Journal·Nuria RoldanBegoña García-Álvarez
Oct 14, 2011·Physical Chemistry Chemical Physics : PCCP·Moutusi Manna, Chaitali Mukhopadhyay
Jan 26, 2017·Frontiers in Cell and Developmental Biology·Pushpendra Singh
Dec 26, 2018·Biophysical Journal·Thomas K M NyholmJ Peter Slotte
Apr 15, 2021·The Plant Journal : for Cell and Molecular Biology·Linhui YuChangcheng Xu
Mar 24, 2011·The Journal of Physical Chemistry. B·Heather A Pillman, G J Blanchard
Oct 27, 2018·Chemical Reviews·Marek CebecauerMartin Hof
Aug 20, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Oskar EngbergDaniel Huster

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