Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM(TM)): application to lipid-specific membrane protein topogenesis

Methods : a Companion to Methods in Enzymology
Mikhail BogdanovWilliam Dowhan

Abstract

We provide an overview of lipid-dependent polytopic membrane protein topogenesis, with particular emphasis on Escherichia coli strains genetically altered in their lipid composition and strategies for experimentally determining the transmembrane organization of proteins. A variety of reagents and experimental strategies are described including the use of lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by substituted cysteine site-directed chemical labeling. Employing strains in which lipid composition can be controlled temporally during membrane protein synthesis and assembly provides a means to observe dynamic changes in protein topology as a function of membrane lipid composition.

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Citations

May 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Caralyn E FlackJovanka M Voyich
May 8, 2007·PLoS Computational Biology·Tristan UrsellRob Phillips
Jun 19, 2007·Journal of Bacteriology·Joeli Marrero, Matthew K Waldor
Nov 5, 2008·Journal of Lipid Research·William Dowhan
Nov 5, 2008·The Journal of Biological Chemistry·Malin WikströmAke Wieslander
Dec 17, 2008·The Journal of Biological Chemistry·Mikhail BogdanovWilliam Dowhan
Jun 6, 2009·Annual Review of Biochemistry·William Dowhan, Mikhail Bogdanov
Jun 6, 2009·The Journal of Biological Chemistry·Hseuh-Liang FuBarry P Rosen
Dec 29, 2009·The Journal of Biological Chemistry·Cassandra S Arendt, Buddy Ullman
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mikhail BogdanovWilliam Dowhan
Jul 27, 2012·Neuroscience Bulletin·Wei-Guang Li, Tian-Le Xu
Mar 14, 2012·Journal of the American Society for Mass Spectrometry·Yan PanLars Konermann
Dec 1, 2012·Expert Review of Proteomics·Lars Konermann, Yan Pan
Aug 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Christian Monzel, Gottfried Unden
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