Transmembrane signaling in chimeras of the Escherichia coli aspartate and serine chemotaxis receptors and bacterial class III adenylyl cyclases.

The Journal of Biological Chemistry
Kajal KanchanJoachim E Schultz

Abstract

The Escherichia coli chemoreceptors for serine (Tsr) and aspartate (Tar) and several bacterial class III adenylyl cyclases (ACs) share a common molecular architecture; that is, a membrane anchor that is linked via a cytoplasmic HAMP domain to a C-terminal signal output unit. Functionality of both proteins requires homodimerization. The chemotaxis receptors are well characterized, whereas the typical hexahelical membrane anchor (6TM) of class III ACs, suggested to operate as a channel or transporter, has no known function beyond a membrane anchor. We joined the intramolecular networks of Tsr or Tar and two bacterial ACs, Rv3645 from Mycobacterium tuberculosis and CyaG from Arthrospira platensis, across their signal transmission sites, connecting the chemotaxis receptors via different HAMP domains to the catalytic AC domains. AC activity in the chimeras was inhibited by micromolar concentrations of l-serine or l-aspartate in vitro and in vivo. Single point mutations known to abolish ligand binding in Tar (R69E or T154I) or Tsr (R69E or T156K) abrogated AC regulation. Co-expression of mutant pairs, which functionally complement each other, restored regulation in vitro and in vivo. Taken together, these studies demonstrate chemotax...Continue Reading

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Citations

Sep 21, 2013·Trends in Biochemical Sciences·Joachim E Schultz, Janani Natarajan
Apr 19, 2012·The Journal of Biological Chemistry·Jihong WangJohn L Spudich
Mar 20, 2012·The Journal of Biological Chemistry·Karin WinklerJoachim E Schultz
Jul 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Min-Hyung RyuMark Gomelsky
Mar 15, 2011·Structure·Hedda U FerrisMurray Coles
Apr 1, 2014·Journal of Structural Biology·Hedda U FerrisMarcus D Hartmann
Jan 18, 2015·International Journal of Medical Microbiology : IJMM·Joachim E SchultzMiriam Ziegler
Jan 9, 2008·PLoS Computational Biology·Clinton H HansenNed S Wingreen
Sep 8, 2004·Trends in Cell Biology·Daniel J WebreJeffry B Stock
Nov 19, 2011·The Journal of Biological Chemistry·Laura García MondéjarJoachim E Schultz

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