PMID: 9421967Jan 9, 1998Paper

Transmembrane signaling mediated by water in bovine rhodopsin

Photochemistry and Photobiology
S NishimuraA Maeda

Abstract

Unhydrated air-dried films of rhodopsin from bovine rod outer segment membranes do not produce its active state, metarhodopsin II. In order to reveal requirements for its formation, we studied changes in H-bonding of water, peptide carbonyl and carboxylic acid in the photochemical reactions by means of difference Fourier transform infrared spectroscopy, under both hydrated and unhydrated conditions. A water molecule near Glu113, which undergoes H-bonding change in bathorhodopsin, remained in the unhydrated film, but with a weaker H-bonding state than in the hydrated film. The other water molecules, which shfit in lumirhodopsin and metarhodopsin I as well as in bathorhodopsin of the hydrated film, were not observed in the unhydrated film. Effects of the dehydration were detected in all the C=O stretching vibrations of the peptide backbone and of Asp83 in the formation of bathorhodopsin. The C=O stretching band of Asp83 of lumirhodopsin and metarhodopsin I is intensified in the unhydrated film. We propose that structural changes at the intradiscal site in the interaction between the Schiff base and Glu113 affect water molecules, the peptide backbone, Asp83 and Glu122 in helices B and C through consecutive photochemical processes ...Continue Reading

References

Sep 1, 1995·Biophysical Chemistry·G L DeCaluwéW J de Grip
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Arnis, K P Hofmann
Apr 22, 1993·Nature·G F SchertlerR Henderson

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Citations

Feb 25, 2003·Chemistry and Physics of Lipids·Amitabha Chattopadhyay
Sep 14, 2000·Biochimica Et Biophysica Acta·H Kandori

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