Transmembrane topography of subunit a in the Escherichia coli F1F0 ATP synthase.

The Journal of Biological Chemistry
F I Valiyaveetil, R H Fillingame

Abstract

Subunit a is the least understood of the three subunits that compose the F0 sector in the Escherichia coli F0F1 ATP synthase. In this study, we have substituted Cys into predicted extramembranous loops of the protein and used chemical modification to obtain topographical information on the folding of subunit a. The extent of labeling of the substituted Cys residues by fluorescein-5'-maleimide was determined. The localization of reactive Cys residues was inferred from differences in the extent of labeling in inside out and right side out membrane vesicles. The NH2-terminal segment of subunit a was localized to the outside (periplasmic) surface and the COOH terminus to the cytoplasmic surface by these procedures. Loop residues in two periplasmic extramembranous loops and in two cytoplasmic extramembranous loops were also localized. The localization of two cytoplasmic Cys residues was confirmed by using 4-acetamido-4'-maleimidylstilbene-2,2'-disulfonic acid to block fluorescein-5'-maleimide labeling. From the localization of the Cys residues, a model for the topography is proposed that consists of five transmembrane segments with the NH2 terminus periplasmic and the COOH terminus cytoplasmic. The positions of second site suppresso...Continue Reading

References

Jan 1, 1988·Physiological Reviews·A E Senior
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S A ShortS C Silverstein
Sep 6, 1984·Biochimica Et Biophysica Acta·J E WalkerN J Gay
Aug 11, 1995·Journal of Molecular Biology·J ZhouD F Blair
Jan 13, 1995·The Journal of Biological Chemistry·T W Loo, D M Clarke
Feb 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·M BorrásG Leclercq
May 1, 1996·The Journal of Membrane Biology·R K Nakamoto
Jan 1, 1996·Annual Review of Biophysics and Biomolecular Structure·B M Conti-FineK E Mclane
Jan 1, 1996·Annual Review of Microbiology·G Deckers-Hebestreit, K Altendorf
Jan 24, 1998·The Journal of Biological Chemistry·F I Valiyaveetil, R H Fillingame

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Citations

Jan 18, 2007·Plant Cell Reports·Hong ZhangYingguo Zhu
May 13, 2006·Journal of Bioenergetics and Biomembranes·Steven B Vik, Robert R Ishmukhametov
Dec 15, 2000·Current Opinion in Structural Biology·D StockJ E Walker
Jun 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jonna K HakulinenThomas Meier
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lindsay A BakerJohn L Rubinstein
Jun 23, 2009·The Journal of Biological Chemistry·P Ryan Steed, Robert H Fillingame
May 9, 2009·The Journal of Biological Chemistry·Melanie VollmarGeorg Groth
Mar 14, 2008·The Journal of Biological Chemistry·Kyle J MooreRobert H Fillingame
Sep 13, 2008·The Journal of Biological Chemistry·Monika G DüserMichael Börsch
Oct 17, 1998·The EMBO Journal·G Kaim, P Dimroth
Jun 5, 1999·The EMBO Journal·A KiharaK Ito
Apr 29, 2011·Journal of Biochemistry·Daichi OkunoHiroyuki Noji
May 23, 2002·Antimicrobial Agents and Chemotherapy·Antonio Javier Martín-GalianoAdela G de la Campa
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·M van Geest, J S Lolkema
Jun 6, 2009·Annual Review of Biochemistry·Christoph von BallmoosPeter Dimroth
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·R K NakamotoM K al-Shawi
Aug 7, 2010·PLoS Biology·Laura PreissThomas Meier
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christine M AngevineRobert H Fillingame
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·P DimrothG Oster
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Kawasaki-NishiM Forgac
Dec 17, 2015·Trends in Biochemical Sciences·Werner Kühlbrandt, Karen M Davies
Aug 1, 2009·The EMBO Journal·Monika G DüserMichael Börsch
Dec 14, 2011·Protein Science : a Publication of the Protein Society·Eva-Maria E UhlemannOleg Y Dmitriev
Jan 30, 2013·Biochemical Society Transactions·John E Walker
May 30, 2006·Biochimica Et Biophysica Acta·Joachim Weber
Nov 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·P Ryan SteedRobert H Fillingame
Nov 9, 2016·The Journal of General Physiology·Vanessa Leone, José D Faraldo-Gómez
Jun 3, 2008·Archives of Biochemistry and Biophysics·Robert K NakamotoMarwan K Al-Shawi

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