Transmembrane topology of the Rieske Fe/S protein of the cytochrome b6/f complex from spinach chloroplasts

FEBS Letters
I KarnauchovR B Klösgen

Abstract

The topology of the Rieske protein of the cytochrome b6/f complex in thylakoids from spinach chloroplasts was examined by protease protection experiments as well as polypeptide extraction assays using solutions of chaotropic salts or alkaline pH. While neither thermolysin nor trypsin cleave any of the Rieske protein when added to the stromal side of the thylakoid membrane, proteinase K is capable of removing approximately four residues from its NH2-terminus. The protein is resistant to membrane extraction by 0.1 M Na2CO3 or 2 M NaBr but is quantitatively released by 0.1 M NaOH. Treatment of thylakoids with 2 M NaSCN leads to extraction of variable amounts of the protein, depending on the presence or absence of sucrose in the medium which apparently stabilizes the cytochrome complex. From these results we conclude that the Rieske protein is an integral component of the cytochrome complex which spans the thylakoid membrane with a single hydrophobic segment and is anchored predominantly by electrostatic interactions.

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Citations

Jun 11, 2002·Biochemical and Biophysical Research Communications·Jürgen M SteinerWolfgang Löffelhardt
Feb 13, 2001·European Journal of Biochemistry·R M MouldJ C Gray
Oct 10, 2008·The Journal of Biological Chemistry·Stefan FrielingsdorfRalf Bernd Klösgen
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·N ShakibaiL Rothfield
Nov 1, 2007·Molecular Plant Pathology·Yuhong ShiMichael J Adams
Dec 14, 2005·Journal of Molecular Biology·Bo HouRalf Bernd Klösgen
Jan 3, 2006·Journal of Plant Physiology·Michael GutensohnRalf Bernd Klösgen
Nov 23, 2017·BMC Plant Biology·Jarosław KróliczewskiBożena Króliczewska
Dec 20, 2007·The Plant Journal : for Cell and Molecular Biology·Hironari NomuraTakashi Shiina
Jun 22, 2007·The Journal of Biological Chemistry·Stefan Frielingsdorf, Ralf Bernd Klösgen
Jan 15, 2002·The Journal of Biological Chemistry·Dirk SchneiderMatthias Rögner
Feb 7, 2006·Biochimica Et Biophysica Acta·Beata GubernatorAndrzej Szczepaniak
Mar 20, 2019·ACS Synthetic Biology·James B Y H BehrendorffMathias Pribil

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