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Transmethylation and transguanylylation in 5'-RNA capping system isolated from rat liver nuclei

Proceedings of the National Academy of Sciences of the United States of America

Oct 1, 1979

K Mizumoto, F Lipmann

PMID: 291913

Abstract

Rat liver nuclei were isolated and sonicated for extraction in order to study the capping of RNA. The guanosine 7-methyltransferase was purified from the extract by hydroxylapatite column chromatography with stepwise addition of phosphate buffer. It was assayed by using as methyl accept...read more

Mentioned in this Paper

Guanine
Guanosine
Sephadex G
Phosphate buffers
Cap Formation
Chromatography
Polynucleotides
Dithiothreitol
RNA Capping
Cell Nucleus
Paper Details
References
  • References9
  • Citations30
1
  • References9
  • Citations30
123

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Transmethylation and transguanylylation in 5'-RNA capping system isolated from rat liver nuclei

Proceedings of the National Academy of Sciences of the United States of America

Oct 1, 1979

K Mizumoto, F Lipmann

PMID: 291913

DOI:

Abstract

Rat liver nuclei were isolated and sonicated for extraction in order to study the capping of RNA. The guanosine 7-methyltransferase was purified from the extract by hydroxylapatite column chromatography with stepwise addition of phosphate buffer. It was assayed by using as methyl accept...read more

Mentioned in this Paper

Guanine
Guanosine
Sephadex G
Phosphate buffers
Cap Formation
Chromatography
Polynucleotides
Dithiothreitol
RNA Capping
Cell Nucleus

Similar Papers Found In These Feeds

mRNA capping

mRNA capping, the addition of a 5’ 7-methylguanosine cap to messenger RNA, is an evolutionarily conserved process. Here is the latest research on mRNA capping enzymes and the importance of mRNA capping on biological processes.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America

5'-Terminal capping of RNA by guanylyltransferase from HeLa cell nuclei

Proceedings of the National Academy of Sciences of the United States of AmericaSeptember 1, 1977
C Wei, B Moss
Proceedings of the National Academy of Sciences of the United States of America

Mechanism of mRNA capping by vaccinia virus guanylyltransferase: characterization of an enzyme--guanylate intermediate

Proceedings of the National Academy of Sciences of the United States of AmericaJanuary 1, 1981
S Shuman, J Hurwitz
Paper Details
References
  • References9
  • Citations30
1
  • References9
  • Citations30
123
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