Transmissible gastroenteritis virus infection induces apoptosis through FasL- and mitochondria-mediated pathways.

Veterinary Microbiology
Li DingDewen Tong

Abstract

Transmissible gastroenteritis virus (TGEV) has been reported to induce apoptosis in swine testis (ST) cells. However, the mechanisms underlying TGEV-induced apoptosis are still unclear. In this study we observed that TGEV infection induced apoptosis in porcine kidney (PK-15) cells in a time- and dose-dependent manner. TGEV infection up-regulated FasL, activated FasL-mediated apoptotic pathway, leading to activation of caspase-8 and cleavage of Bid. In addition, TGEV infection down-regulated Bcl-2, up-regulated Bax expression, promoted translocation of Bax to mitochondria, activated mitochondria-mediated apoptotic pathway, which in turn caused the release of cytochrome c and the activation of caspase-9. Both extrinsic and intrinsic pathways activated downstream effector caspase-3, followed by the cleavage of PARP, resulting in cell apoptosis. Moreover, TGEV infection did not induce significant DNA fragmentation in ammonium chloride (NH(4)Cl) pretreated PK-15 cells or cells infected with UV-inactivated TGEV. In turn, block of caspases activation also did not affect TGEV replication. Taken together, this study demonstrates that TGEV-induced apoptosis is dependent on viral replication in PK-15 cells and occurs through activation of...Continue Reading

References

Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·E H ChengJ M Hardwick
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Feb 4, 1999·Archives of Virology·T SirinarumitrP S Paul
Apr 28, 1999·Trends in Microbiology·H Everett, G McFadden
Dec 28, 1999·Annual Review of Cell and Developmental Biology·R H Arch, C B Thompson
Aug 10, 2000·Journal of Virology·P ClarkeK L Tyler
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 23, 2004·Virology·Wen L ShihHung J Liu
Feb 6, 2007·Virus Research·Nicola DecaroCanio Buonavoglia
Aug 1, 2008·Journal of Virology·Alessio LorussoRaoul J de Groot
Jun 13, 2009·Apoptosis : an International Journal on Programmed Cell Death·Chi-Hung LinHung-Jeng Liu
Mar 30, 2010·Veterinary Microbiology·Channakeshava Sokke UmeshappaAwadh Bihari Pandey
Feb 9, 2012·Advances in Virology·Annamaria Pratelli

❮ Previous
Next ❯

Citations

Jan 11, 2016·Wiley Interdisciplinary Reviews. RNA·Binbin DingMingzhou Chen
Nov 15, 2013·Biochemical and Biophysical Research Communications·Li DingDewen Tong
Dec 17, 2014·Biochemical and Biophysical Research Communications·Hongling ZhangDewen Tong
Feb 20, 2014·Biochemical and Biophysical Research Communications·Li DingDewen Tong
Feb 28, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ximei LeiChuanqing Wang
Sep 27, 2018·The Journal of Veterinary Medical Science·Li DingMeiling Hong
Sep 28, 2018·Molecular & Cellular Proteomics : MCP·Xiaomin ZhaoDewen Tong
Jan 1, 2019·Molecular & Cellular Proteomics : MCP·Xiaomin ZhaoDewen Tong
Mar 4, 2021·Life·Katerina GiotiApostolos Beloukas
Jun 3, 2021·Journal of Animal Physiology and Animal Nutrition·Xiuli LiangWenju Zhang
Aug 11, 2021·European Journal of Medicinal Chemistry·Yang HuJiong Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
Protein Assay
PCR
fluorescence microscopy
X-ray

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.