Transmission and microevolution of USA300 MRSA in U.S. households: evidence from whole-genome sequencing

MBio
Md Tauqeer AlamMichael Z David

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) USA300 is a successful S. aureus clone in the United States and a common cause of skin and soft tissue infections (SSTIs). We performed whole-genome sequencing (WGS) of 146 USA300 MRSA isolates from SSTIs and colonization cultures obtained from an investigation conducted from 2008 to 2010 in Chicago and Los Angeles households that included an index case with an S. aureus SSTI. Identifying unique single nucleotide polymorphisms (SNPs) and analyzing whole-genome phylogeny, we characterized isolates to understand transmission dynamics, genetic relatedness, and microevolution of USA300 MRSA within the households. We also compared the 146 USA300 MRSA isolates from our study with the previously published genome sequences of the USA300 MRSA isolates from San Diego (n = 35) and New York City (n = 277). We found little genetic variation within the USA300 MRSA household isolates from Los Angeles (mean number of SNPs ± standard deviation, 17.6 ± 35; π nucleotide diversity, 3.1 × 10(-5)) or from Chicago (mean number of SNPs ± standard deviation, 12 ± 19; π nucleotide diversity, 3.1 × 10(-5)). The isolates within a household clustered into closely related monophyletic groups, suggesting the...Continue Reading

References

Mar 5, 1998·JAMA : the Journal of the American Medical Association·B C HeroldR S Daum
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Sep 4, 2003·Genome Research·Li LiDavid S Roos
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Oct 30, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Erica S PanFrançoise Perdreau-Remington
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jan 14, 2005·Bioinformatics·Federico AbascalDavid Posada
Apr 6, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Timothy F JonesWilliam Schaffner
Aug 18, 2006·The New England Journal of Medicine·Gregory J MoranUNKNOWN EMERGEncy ID Net Study Group
Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Adam D KennedyFrank R DeLeo
Mar 20, 2008·Genome Research·Daniel R Zerbino, Ewan Birney
Feb 24, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ari RobicsekLance R Peterson
Mar 27, 2009·Journal of Clinical Microbiology·Brandi LimbagoUNKNOWN Active Bacterial Core surveillance MRSA Investigators
Jun 6, 2009·Bioinformatics·Samuel AssefaMatthew Berriman
Jun 10, 2009·Bioinformatics·Salvador Capella-GutiérrezToni Gabaldón
Jul 18, 2009·The Journal of Antimicrobial Chemotherapy·Fred C Tenover, Richard V Goering
Jan 19, 2010·Bioinformatics·Heng Li, Richard Durbin
Jan 23, 2010·Science·Simon R HarrisStephen D Bentley
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Jun 22, 2011·Briefings in Bioinformatics·David M KristensenEugene V Koonin
Jun 22, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David A TalanUNKNOWN EMERGEncy ID Net Study Group
Feb 2, 2012·Genome Research·Nicola CasaliFrancis Drobniewski
Mar 1, 2012·Molecular Biology and Evolution·Alexei J DrummondAndrew Rambaut
Apr 5, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Loren G MillerRobert S Daum
May 25, 2012·BMC Genomics·Jason R GrantPaul Stothard
Jul 26, 2012·BMC Genomics·Michael InouyeKathryn E Holt
Aug 1, 2012·Nature Methods·Diego DarribaDavid Posada
Oct 30, 2012·The Lancet Infectious Diseases·Laura J ShallcrossAndrew C Hayward
May 30, 2013·Journal of the Pediatric Infectious Diseases Society·Marcela RodriguezStephanie A Fritz
Oct 8, 2013·Applied and Environmental Microbiology·Bruno Contreras-Moreira, Pablo Vinuesa
Oct 24, 2013·The Journal of Antimicrobial Chemotherapy·Birgit StrommengerUlrich Nübel

❮ Previous
Next ❯

Citations

Apr 14, 2015·Trends in Microbiology·Justin KnoxFranklin D Lowy
Sep 9, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kyle J PopovichRobert A Weinstein
May 6, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J-M RolainD Raoult
May 22, 2016·Infectious Disease Clinics of North America·David van Duin, David L Paterson
Jun 12, 2016·Proceedings of the National Academy of Sciences of the United States of America·Emanuele BosiBernhard Ø Palsson
Sep 30, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E Berla-KerzhnerD Glikman
Apr 19, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Eugene V MillarKimberly A Bishop-Lilly
Dec 1, 2017·British Medical Bulletin·Andrew Henderson, Graeme R Nimmo
Jun 23, 2018·Epidemiology and Infection·B M HansonT C Smith
Mar 5, 2016·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·O Colin StineMary-Claire Roghmann
Apr 23, 2019·PLoS Computational Biology·Marko JärvenpääPekka Marttinen
May 16, 2015·Nature Reviews. Microbiology·Alison E Mather
Jun 7, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Taj Azarian
Apr 5, 2017·The Journal of Infectious Diseases·Paul J Planet
Sep 27, 2018·Antimicrobial Resistance and Infection Control·Kevin T KavanaghLindsay E Calderon
Jun 28, 2019·Antimicrobial Resistance and Infection Control·Kevin T Kavanagh
Sep 8, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jorunn Pauline CavanaghElizabeth G Aarag Fredheim
Mar 30, 2017·Microbial Genomics·Dorota M JamrozyMatthew T G Holden
Jun 15, 2018·Proceedings. Biological Sciences·Beth GibsonAdam Eyre-Walker
Feb 10, 2019·Nature Reviews. Microbiology·Nicholas A TurnerVance G Fowler
Aug 15, 2019·Implementation Science : IS·Nina Van GoethemAnnie Robert
Aug 3, 2016·Annual Review of Microbiology·J Ross Fitzgerald, Matthew T G Holden
Dec 7, 2018·BMC Microbiology·Timothy D ReadMichael Z David
Mar 12, 2020·Frontiers in Microbiology·Miklos FuziAkos Toth

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Datasets Mentioned

BETA
SRP039020

Methods Mentioned

BETA
electrophoresis
genotyping

Software Mentioned

GET
LogCombiner
Bioconductor library
R heatmap2
Prokka
Genome Analysis Toolkit UnifiedGenotyper
RAxML
TreeAnnotator
Gen
REALPHY

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