Transmission electron microscopy and light scattering studies on the interaction of a nonionic/anionic surfactant mixture with phosphatidylcholine liposomes

Microscopy Research and Technique
A de la MazaJ L Parra

Abstract

The interaction of an equimolecular mixture of nonylphenol polyethoxylated [NP(EO)10] and sodium dodecyl sulfate (SDS) surfactants with phosphatidylcholine (PC) liposomes was studied by means of transmission electron microscopy (TEM) and changes in the mean particle size (quasielastic light scattering; QELS) and in the static light scattering (SLS) of the system during liposome solubilization. A good correlation was found between the TEM diameter of particles and the mean hydrodynamic diameter (HD) determined by QELS. The aggregates resulting in this interaction were dependent on the surfactant concentration in the system. Thus, an initial vesicle growth occurred when the surfactant concentration was 15.98 mol%, together with the formation of a very small percentage of smaller particles. Additional surfactant amounts (28.32 mol%) led first to the formation of largest vesicles (HD 418 nm) and second to a fall in the vesicle diameter and in the SLS of the system. Thus, for 38.27 mol%, the TEM picture still showed the presence of vesicles, albeit with signs of disintegration. When additional amounts of surfactant were added to the system, the size curve started to show a bimodal distribution. Thus, for 51.81 mol% surfactant concen...Continue Reading

References

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Nov 1, 1994·The Biochemical Journal·A De la Maza, J L Parra

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Citations

May 10, 2005·American Journal of Respiratory and Critical Care Medicine·Ross RidsdaleMartin Post
Nov 7, 2006·Biochimica Et Biophysica Acta·Dmitry VolodkinHelmuth Mohwald
Oct 8, 2003·BioFactors·Hironori TsuchiyaHiroshi Takeuchi
Nov 18, 1998·Journal of Microencapsulation
Jul 12, 2005·Biochimica Et Biophysica Acta·S SennatoP Malaspina
Jul 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·F BordiS Sennato

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