Transmission electron microscopy comparison of methods for collecting in situ formed enamel pellicle

Clinical Oral Investigations
M HannigY Açil

Abstract

The in vivo formed salivary pellicle is composed of an outer globular and a densely structured basal layer. This study developed a method for selective recovering of these pellicle layers from the enamel surface. Two-hour in situ pellicles were formed by intraoral exposure of enamel specimens in two adults. Pellicle-covered enamel specimens were treated either mechanically (scraping with scaler, curette or razor blade, or rubbing with a sponge) or chemically (phosphate buffer, NaCl, NaOCl, CaCl2, NaSCN, urea, tetrahydrofurane, guanidine, SDS, HCl, or EDTA with or without additional ultrasonication). Specimens were processed for transmission electron microscopic analysis to detect pellicle residues remaining on the enamel surface after the different treatments. Most of the chemical treatments caused partial, incomplete removal of the globular layer. Complete removal of the globular layer without disruption of the basal layer was obtained by sponge rubbing or by CaCl2 combined with ultrasonication, whereas scraping caused partial disruption of the basal layer. Removal of the basal layer was observed after treatment with HCl, EDTA, or NaOCl combined with ultrasonication. Electrophoretical analysis of recovered pellicle fractions i...Continue Reading

References

May 1, 1977·Scandinavian Journal of Dental Research·T Lie
Jun 1, 1992·FEMS Microbiology Reviews·H J BusscherH C van der Mei
Oct 1, 1991·Acta Odontologica Scandinavica·T Arnebrant, T Simonsson
Apr 1, 1990·Scandinavian Journal of Dental Research·M RykkeG Rölla
Jan 1, 1989·Archives of Oral Biology·I Al-Hashimi, M J Levine
Aug 1, 1987·Scandinavian Journal of Dental Research·B Nyvad, O Fejerskov
Jan 1, 1986·Archives of Oral Biology·G EmberyJ B Stanbury
Aug 1, 1967·Archives of Oral Biology·D I Hay
Jan 1, 1967·Caries Research·S A LeachC A Saxton
Jan 1, 1967·Caries Research·W G Armstrong
Jun 1, 1993·Scandinavian Journal of Dental Research·N VassilakosP O Glantz
Dec 1, 1995·Acta Odontologica Scandinavica·K K SkjørlandT Sønju
Feb 26, 1998·Acta Odontologica Scandinavica·A B Sønju ClasenT Sønju
Aug 26, 1999·Caries Research·M Hannig, M Balz
Mar 10, 2000·Archives of Oral Biology·A M Vacca Smith, W H Bowen
Mar 29, 2001·Archives of Oral Biology·Y YaoF G Oppenheim
Feb 15, 2002·Advances in Dental Research·U LendenmannF G Oppenheim
Dec 24, 2003·Journal of Dental Research·J LiF G Oppenheim
Jul 29, 2004·European Journal of Oral Sciences·Ljubomir VitkovWolf Dietrich Krautgartner

❮ Previous
Next ❯

Citations

Jan 1, 1973·Preparative Biochemistry·R C BoltzM K Louie
Jan 1, 1977·Preparative Biochemistry·W S Bont, J H Hilgers
Jul 26, 2012·Journal of Lipid Research·Marco ReichKlaus Kümmerer
Oct 25, 2008·The Angle Orthodontist·Selma Elekdag-TurkTamer Turk
Jul 1, 2008·Journal of Oral Science·Susumu AndoMasashi Miyazaki
Mar 16, 2007·Clinical Oral Investigations·Daniela DeimlingMatthias Hannig
Dec 8, 2009·Clinical Oral Investigations·Christian HannigMatthias Hannig
Oct 12, 2012·Clinical Oral Investigations·A KenscheC Hannig
Jan 20, 2011·Analytical and Bioanalytical Chemistry·Christine MüllerChristiane Ziegler
Dec 20, 2007·Acta Odontologica Scandinavica·Lene Hystad HoveAnne Bjørg Tveit
Aug 19, 2011·Journal of Endodontics·Christian SengesAli Al-Ahmad
Mar 10, 2010·Journal of Microbiological Methods·David Jonathan JungChristian Hannig
Apr 28, 2009·Journal of Dentistry·Christian HannigAli Al-Ahmad
Mar 27, 2009·Archives of Oral Biology·C HannigM Hannig
Apr 22, 2008·Archives of Oral Biology·Christian HannigMatthias Hannig
Mar 1, 2015·Colloids and Surfaces. B, Biointerfaces·Fabian KratzChristiane Ziegler
Jun 2, 2006·Journal of Biomedical Materials Research. Part a·Christian HannigThomas Attin
Sep 1, 2015·European Journal of Oral Sciences·Susann GrychtolChristian Hannig
Jun 23, 2005·Archives of Oral Biology·Christian HannigThomas Attin
Mar 26, 2015·Archives of Oral Biology·Kássia Regina Simões MeiraFrederico Barbosa de Sousa
Sep 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Javier SotresThomas Arnebrant
Mar 4, 2020·Clinical Oral Investigations·Miryam Martínez-HernándezMatthias Hannig
Dec 1, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Miryam Martínez-HernándezArgelia Almaguer-Flores
Jan 19, 2021·Colloids and Surfaces. B, Biointerfaces·Nicholas G Fischer, Conrado Aparicio
Jul 3, 2021·Journal of Clinical Medicine·Chen-Xuan WeiW Keung Leung
Jul 30, 2021·Archives of Oral Biology·Wadim RasputnisMatthias Hannig
Jan 9, 2007·Biomacromolecules·Marité CárdenasLiselott Lindh
Apr 24, 2007·Journal of Proteome Research·Walter L SiqueiraFrank G Oppenheim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.