Transmission electron microscopy (TEM) of plant tissues

Cold Spring Harbor Protocols
Martin HülskampHeinz Schwarz

Abstract

Transmission electron microscopy (TEM) requires extensive preparation of plant tissues to achieve optimal results. Samples must be fixed to preserve ultrastructure, dehydrated to withstand the high vacuum of an electron microscope, and embedded in a suitable resin to permit very thin sections to be produced. This protocol provides details for fixation, dehydration with ethanol, and embedding in Spurr's, an epoxide resin with low viscosity that is well-suited for plant tissues.

References

Jul 22, 2010·Cold Spring Harbor Protocols·Detlef Weigel, Jane Glazebrook

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Citations

May 9, 2014·The Plant Journal : for Cell and Molecular Biology·Myat T LinMaureen R Hanson
Jun 25, 2015·Environmental Science & Technology·John P StegemeierGregory V Lowry
Jul 22, 2010·Cold Spring Harbor Protocols·Detlef Weigel, Jane Glazebrook

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