Transmission of an oxygen availability signal at the Salmonella enterica serovar Typhimurium fis promoter

PloS One
Andrew D S CameronCharles J Dorman

Abstract

The nucleoid-associated protein FIS is a global regulator of gene expression and chromosome structure in Escherichia coli and Salmonella enterica. Despite the importance of FIS for infection and intracellular invasion, very little is known about the regulation of S. enterica fis expression. Under standard laboratory growth conditions, fis is highly expressed during rapid growth but is then silenced as growth slows. However, if cells are cultured in non-aerated conditions, fis expression is sustained during stationary phase. This led us to test whether the redox-sensing transcription factors ArcA and FNR regulate S. enterica fis. Deletion of FNR had no detectable effect, whereas deletion of ArcA had the unexpected effect of further elevating fis expression in stationary phase. ArcA required RpoS for induction of fis expression, suggesting that ArcA indirectly affects fis expression. Other putative regulators were found to play diverse roles: FIS acted directly as an auto-repressor (as expected), whereas CRP had little direct effect on fis expression. Deleting regions of the fis promoter led to the discovery of a novel anaerobically-induced transcription start site (Pfis-2) upstream of the primary transcription start site (Pfis-1...Continue Reading

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Citations

Nov 2, 2014·Injury Prevention : Journal of the International Society for Child and Adolescent Injury Prevention·Jake Olivier, Scott R Walter
Jul 15, 2015·Nucleic Acids Research·Veneta GerganovaGeorgi Muskhelishvili
Aug 16, 2014·Injury Prevention : Journal of the International Society for Child and Adolescent Injury Prevention·M Kary
Jun 3, 2016·Clinical Science·Charles J DormanMatthew J Dorman
Jul 20, 2019·BMC Molecular and Cell Biology·Charles J Dorman
Feb 5, 2015·The Journal of Biological Chemistry·Veneta GerganovaGeorgi Muskhelishvili

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Methods Mentioned

BETA
PCR
flow cytometry
electrophoresis
RNA-seq

Software Mentioned

GraphPad Prism
WebSIDD

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