Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts

The Journal of Infectious Diseases
Susana N AsinAlexandra L Howell

Abstract

Women can become infected with human immunodeficiency virus type 1 (HIV-1) after the heterosexual transmission of virus from an infected male partner. To understand the events that result in transmission of HIV-1 across the female reproductive tract, we characterized the life-cycle events of HIV-1 in primary cultures of human uterine epithelial cells and stromal fibroblasts. Epithelial cells and stromal fibroblasts released virus particles after exposure to either X4- or R5-tropic strains of HIV-1. Virus released by these cells was able to infect CD4(+) T cells. When exposed to an X4-tropic strain of HIV-1, these cells supported HIV-1 reverse transcription, integration, and viral DNA transcription. When exposed to an R5-tropic strain, however, these cells released unmodified virus. These data suggest that uterine cells are targets for productive infection with X4-tropic strains and release unmodified R5-tropic viruses that would then be able to infect submucosal target cells, including T cells and macrophages.

Citations

Mar 7, 2007·Current HIV/AIDS Reports·Gavin MorrowMelissa Robbiani
Aug 11, 2005·Current HIV/AIDS Reports·Alexandra L HowellCharles R Wira
May 9, 2008·Current Infectious Disease Reports·Gavin MorrowMelissa Robbiani
Nov 4, 2011·Viruses·Stéphanie Durand, Andrea Cimarelli
Jan 11, 2013·Clinical & Developmental Immunology·Johanne PoudrierMichel Roger
Feb 26, 2013·Journal of Reproductive Immunology·Marta Rodriguez-GarciaCharles R Wira
Sep 2, 2005·American Journal of Reproductive Immunology : AJRI·Todd M SchaeferCharles R Wira
Jun 17, 2009·American Journal of Reproductive Immunology : AJRI·Mimi GhoshCharles R Wira
Sep 3, 2011·American Journal of Reproductive Immunology : AJRI·Kimberly D ColemanJohn V Fahey
Jan 13, 2011·American Journal of Reproductive Immunology : AJRI·Barbara L Shacklett, Ruth M Greenblatt
Jan 13, 2011·American Journal of Reproductive Immunology : AJRI·Charu Kaushic
Apr 3, 2014·American Journal of Reproductive Immunology : AJRI·Ruizhong ShenPhillip D Smith
Mar 26, 2014·American Journal of Reproductive Immunology : AJRI·Charles R WiraJohn V Fahey
May 11, 2005·Biochemical and Biophysical Research Communications·Antoine BoucqueyPhilippe Horellou
Oct 15, 2014·BioMed Research International·Juliana Reis MachadoRosana Rosa Miranda Corrêa
Feb 17, 2017·PLoS Pathogens·Jason A NeidlemanNadia R Roan
Aug 17, 2018·Journal of Virology·Jennifer L WelchChioma M Okeoma
Sep 5, 2006·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Junying ZhengShen Pang
Mar 17, 2006·Nature Reviews. Microbiology·Leonid Margolis, Robin Shattock
Jan 27, 2018·PloS One·Krishnakumar DevadasIndira Hewlett
Apr 27, 2021·Critical Reviews in Microbiology·Sriram YandrapallySharmistha Banerjee

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