Transmitted antiretroviral drug resistance and thumb subdomain polymorphisms among newly HIV type 1 diagnosed patients infected with CRF01_AE and CRF07_BC virus in Guangdong Province, China.

AIDS Research and Human Retroviruses
Changfu YangZhenyu Zhu

Abstract

The aim of this study was to elucidate the prevalence of transmitted drug-resistant (TDR) mutations and reverse transcriptase (RT) thumb subdomain polymorphisms in CRF01_AE and CRF07_BC virus among newly diagnosed, therapy-naive HIV-1 patients in Guangdong Province, China. One hundred and sixty-four samples were collected in the Guangzhou Eighth People's Hospital. The entire protease gene and 300 codons of the entry part of the reverse transcriptase were amplified and sequenced. Furthermore, genotypic drug resistance, polymorphisms, and their phylogeny were analyzed. According to eligibility criteria, seven samples were excluded, and 119 of 157 (75.8%) samples (84 CRF01_AE and 35 CRF07_BC) were amplified and sequenced successfully. The prevalence of TDR identified in the present study was 6.7% [8/119, 95% confidence interval (CI) 1.8-11.6%]. Three major resistance mutations, K103N, M184V, and Y188L, each of which caused more than one drug resistance, appeared in only two patients; the prevalence [1.7 % (2/119)] was relatively low. Until now, this is the first observation of the five newly identified accessory mutations, V35T, K43E, V60I, K122E, and E203D, and seven thumb subdomain polymorphisms, A272P, K277R, K281R, T286A, E291...Continue Reading

References

Dec 16, 2005·Cell Research·Jing Yun LiYi Ming Shao
Feb 16, 2007·AIDS·Patricia A CaneUNKNOWN UK collaborative group on HIV Drug Resistance
Oct 9, 2007·AIDS Patient Care and STDs·Monica M ParkerUNKNOWN Resistance Study Group
Oct 11, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Ju-Yeon ChoiSung Soon Kim
Nov 7, 2008·The Tohoku Journal of Experimental Medicine·Chang Oh KimJune Myung Kim
Jun 10, 2009·The Journal of Antimicrobial Chemotherapy·César GarrigaUNKNOWN Spanish Group for the Study of Antiretroviral Drug Resistance
Feb 6, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Lingjie LiaoYiming Shao

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Datasets Mentioned

BETA
AF197341
AF286230
AF103818

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ContigExpress
BioEdit
Molecular Evolutionary Genetic Analysis
Statistical Package for the Social Sciences ( SPSS
MEGA

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