Transovarial Transmission of Co-Existing Orientia tsutsugamushi Genotypes in Laboratory-Reared Leptotrombidium imphalum

Vector Borne and Zoonotic Diseases
Ratree TakhampunyaAllen L Richards

Abstract

The co-evolution of Orientia tsutsugamushi and its vector/host Leptotrombidium mites is important for this bacterium to survive and exist in its environment. The data in this study demonstrated that O. tsutsugamushi has adapted to take advantage of the parasitic nature of the host's larval stage and thus increase its chance of transmission to a vertebrate host and potentially to other vector mites by increasing its density at the time of transmission. Our data demonstrated that during the larval stage the density of O. tsutsugamushi was at its highest level compared to other life stages (Kruskal-Wallis, p < 0.0001). We further revealed that the different O. tsutsugamushi 56-kDa type-specific antigen (TSA) genotypes within the mite were maintained and preserved during transovarial transmission from the Leptotrombidium imphalum, lines Li-3 and Li-5. No sequence difference of 56-kDa TSA gene (variable domain I-III, 765 bp) was observed between the UT302-like genotype found in mothers and their offspring (100% identity). However, one or two nonsynonymous mutations in the 56-kDa TSA gene were observed in the Karp-like genotypes found in the F1 offspring with a percent difference ranging from 0.13 to 0.26 for nucleotide sequences and...Continue Reading

References

Mar 1, 1982·The American Journal of Tropical Medicine and Hygiene·A ShiraiE Gan
Apr 1, 1997·Parasitology Today·R Rosenberg
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Nov 7, 2019·Parasites & Vectors·Ivo ElliottPaul N Newton
Nov 28, 2017·Molecular Microbiology·Christian OttenJeanne Salje
Oct 31, 2020·Tropical Medicine and Infectious Disease·Daniel H ParisAllen L Richards
Mar 28, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ivo ElliottElizabeth M Batty

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Methods Mentioned

BETA
electron microscopy
genotyping
PCR

Software Mentioned

ClustalW
GraphPad Prism
MEGA
ABI 7500 System SDS

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