Transparent carbon ultramicroelectrode arrays: figures of merit for quantitative spectroelectrochemistry for biogenic analysis of reactive oxygen species

Analytical Chemistry
Jonathon DuayKeith J Stevenson

Abstract

Opaque and transparent carbon ultramicro- to nanoelectrode arrays were made using a previously reported facile versatile fabrication method (Duay et al. Anal. Chem. 2014, 86, 11528). First, opaque carbon ultramicroelectrode arrays (CUAs) were characterized for their analytical response to hydrogen peroxide (H2O2) oxidation using cyclic voltammetry. The alumina blocking layer was found to contribute to the noise and thus had undesirable effects on the array's limit of detection (LOD) for H2O2 at fast scan rates. Nonetheless, at slower scan rates (ν ≤ 250 mV s(-1)), the LODs for H2O2 for both opaque (O-CUAs) and transparent arrays (T-CUAs) were found to be lower than previously reported levels for array-based UMEs. LODs as low as 35 nM H2O2 are obtained for T-CUA at a 2.5 mV s(-1) scan rate. Furthermore, the transparent arrays were analyzed for their spectroelectrochemical response during the oxidation/reduction of ferrocenemethanol. Results showed very good correlation between the optical and electrochemical response for ferrocenemethanol at a UV wavelength of 254 nm. Thus, these electrodes allow for the in situ mechanistic and kinetic characterization of heterogeneous electrochemical and intermediate homogeneous chemical reacti...Continue Reading

References

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Nov 16, 2012·Analytical Chemistry·Raphaël TrouillonAndrew G Ewing
Nov 11, 2014·Analytical Chemistry·Jonathon DuayKeith J Stevenson

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Citations

Oct 12, 2019·The Analyst·Olja Simoska, Keith J Stevenson
May 11, 2020·Biotechnology Journal·Francis McEachernGeraldine Merle
Oct 15, 2020·Chemical Reviews·Hui ChenShelley D Minteer
Nov 12, 2020·ACS Sensors·Olja SimoskaKeith J Stevenson

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