Transparent polyvinyl-alcohol cryogel as immobilisation matrix for continuous biohydrogen production by phototrophic bacteria

Biotechnology for Biofuels
Jan-Pierre du Toit, Robert W M Pott

Abstract

Phototrophic purple non-sulfur bacteria (PNSB) have gained attention for their ability to produce a valuable clean energy source in the form biohydrogen via photofermentation of a wide variety of organic wastes. For maturation of these phototrophic bioprocesses towards commercial feasibility, development of suitable immobilisation materials is required to allow continuous production from a stable pool of catalytic biomass in which energy is not diverted towards biomass accumulation, and optimal hydrogen production rates are realised. Here, the application of transparent polyvinyl-alcohol (PVA) cryogel beads to immobilisation of Rhodopseudomonas palustris for long-term hydrogen production is described. PVA cryogel properties are characterised and demonstrated to be well suited to the purpose of continuous photofermentation. Finally, analysis of the long-term biocompatibility of the material is illustrated. The addition of glycerol co-solvent induces favourable light transmission properties in normally opaque PVA cryogels, especially well-suited to the near-infrared light requirements of PNSB. Material characterisation showed high mechanical resilience, low resistance to diffusion of substrates and high biocompatibility of the ma...Continue Reading

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Citations

Jun 3, 2021·Foods·Bahiru TsegayeAmit K Jaiswal
Dec 26, 2021·Biotechnology and Bioengineering·Brandon S Ross, Robert W M Pott
Jan 20, 2022·World Journal of Microbiology & Biotechnology·Genesis Escobedo-MoralesMiriam Paulina Luévanos-Escareño

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Methods Mentioned

BETA
scanning electron microscopy
confocal microscopy

Software Mentioned

Global Analyser Solutions
BioFilmAnalyzer

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