PMID: 2109714Feb 1, 1990Paper

Transplantation analysis of B cell destruction in (NOD x CBA)F1 mouse bone marrow chimeras

Diabetologia
D V SerrezeL D Shultz

Abstract

F1 hybrids produced by outcross of non-obese diabetic (NOD) mice with diabetes resistant strains are also diabetes resistant. This resistance is abrogated if F1 hybrids are lethally irradiated and then haematopoietically reconstituted with NOD bone marrow. This model was employed to determine whether T lymphocyte recognition and elimination of pancreatic B cells in NOD mice is restricted by the MHC haplotype of the target B cell. Diabetes resistant (NOD/Lt x CBA/J)F1 hybrids were lethally irradiated and reconstituted with NOD/Lt bone marrow. Following haematopoietic reconstitution, donor matched NOD/Lt or CBA/J pancreatic islet and anterior pituitary grafts were grafted under a renal capsule to determine whether effector cells derived from NOD/Lt marrow progenitors would reject islet grafts in a MHC restricted fashion. The H-2k haplotype expressed by CBA/J mice differs from all known loci of the unique H-2 haplotype of NOD; therefore, if NOD/Lt T lymphocytes eliminate pancreatic B cells in a MHC restricted fashion. NOD islet grafts would be eliminated in these chimeras while CBA islet grafts would be retained. Overt diabetes developed in 80% of the female and 40% of the male F1 hybrids following reconstitution with NOD/Lt marro...Continue Reading

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Citations

Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·V Thomas-VaslinJ Salaün
Jan 1, 1991·Upsala Journal of Medical Sciences·C HellerströmA Andersson
Apr 28, 2012·European Journal of Immunology·Hermann Wagner
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Clayton E MathewsAlex Rabinovitch
Nov 8, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert L WhitenerClayton E Mathews
Jan 1, 1993·Autoimmunity·A MuirN K MacLaren

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