Transplantation and in vitro perifusion of rat islets of Langerhans after slow cooling and warming in the presence of either glycerol or dimethyl sulfoxide

Cryobiology
M J TaylorP J Davisson

Abstract

The cryoprotectants dimethyl sulfoxide (Me2SO) and glycerol have been used for the cryopreservation of fetal rat pancreases but only Me2SO has been reported for the cryopreservation of adult rat islets. Since glycerol may be preferred to Me2SO for clinical use, this study was undertaken to compare the effectiveness of these cryoprotectants during the slow cooling of isolated adult rat islets. Islets of Langerhans prepared from the pancreases of WAG rats by collagenase digestion were stored at -196 degrees C after slow cooling (0.3 degrees C/min) to -70 degrees C in the presence of multimolar concentrations of either Me2SO or glycerol. Samples were rewarmed slowly (approximately 10 degrees C/min) and dilution of the cryoprotectant was achieved using medium containing sucrose. Function was assessed by determination of the time course of the glucose-induced insulin release during in vitro perifusion at 37 degrees C and also by isograft transplantation. Transplants were carried out by intraportal injection of a minimum of 1700 frozen and thawed islets into streptozotocin-induced diabetic recipients and tissue function was assessed by monitoring blood glucose levels and body weight changes. Without exception the islets frozen and th...Continue Reading

References

Mar 29, 1977·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J Farrant
Mar 1, 1979·Transplantation·P E LacyD W Scharp
Feb 1, 1977·Cryobiology·R V RajotteT K Shnitka
Jan 1, 1976·The Journal of Clinical Investigation·B J FrankelL A Idahl
Aug 1, 1974·Cryobiology·A R HayesR E Kingston
Jan 1, 1980·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R G BretzelK Federlin
Apr 1, 1981·Diabetologia·D E Sutherland

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Citations

Jan 1, 1993·Transplant International : Official Journal of the European Society for Organ Transplantation·J ForemanM J Taylor
Apr 1, 1984·World Journal of Surgery·R V RajotteN N Kneteman
Sep 1, 1991·The Japanese Journal of Surgery·W FukushimaG Nakagawara
Apr 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H L Bank
Feb 1, 1983·Theriogenology·H Lehn-Jensen, W F Rall
Jan 1, 1988·Journal of Immunoassay·H L Bank
Jul 1, 1996·Tissue Engineering·D B Walcerz, A M Karow
May 4, 2016·Journal of Agricultural and Food Chemistry·Charles H Z KongVijayalekshmi Sarojini
Jun 12, 2004·Cell Transplantation·Hesham M El-ShewyEmmanuel C Opara

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