Transplantation of a 3D-printed tracheal graft combined with iPS cell-derived MSCs and chondrocytes.

Scientific Reports
In Gul KimSeong Keun Kwon

Abstract

For successful tracheal reconstruction, tissue-engineered artificial trachea should meet several requirements, such as biocompatible constructs comparable to natural trachea, coverage with ciliated respiratory mucosa, and adequate cartilage remodeling to support a cylindrical structure. Here, we designed an artificial trachea with mechanical properties similar to the native trachea that can enhance the regeneration of tracheal mucosa and cartilage through the optimal combination of a two-layered tubular scaffold and human induced pluripotent stem cell (iPSC)-derived cells. The framework of the artificial trachea was fabricated with electrospun polycaprolactone (PCL) nanofibers (inner) and 3D-printed PCL microfibers (outer). Also, human bronchial epithelial cells (hBECs), iPSC-derived mesenchymal stem cells (iPSC-MSCs), and iPSC-derived chondrocytes (iPSC-Chds) were used to maximize the regeneration of tracheal mucosa and cartilage in vivo. After 2 days of cultivation using a bioreactor system, tissue-engineered artificial tracheas were transplanted into a segmental trachea defect (1.5-cm length) rabbit model. Endoscopy did not reveal granulation ingrowth into tracheal lumen. Alcian blue staining clearly showed the formation of ...Continue Reading

References

Aug 19, 2003·Biomaterials·Jeanie L Drury, David J Mooney
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
May 30, 2006·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Samuel P GubbelsMark K Wax
Aug 12, 2006·The Annals of Otology, Rhinology, and Laryngology·Yukio NomotoKoichi Omori
Jul 24, 2008·The Annals of Otology, Rhinology, and Laryngology·Teruhisa SuzukiKoichi Omori
May 19, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·A Pérez del PalomarM Doblaré
Oct 23, 2010·Nature Biotechnology·Rachel A OldershawSusan J Kimber
Nov 6, 2010·The Annals of Otology, Rhinology, and Laryngology·Mitsuyoshi ImaizumiKoichi Omori
Jan 3, 2012·International Journal of Pediatric Otorhinolaryngology·Xiao Fei DuSeok-Won Park
Mar 24, 2012·Biomaterials·Philipp JungebluthPaolo Macchiarini
Nov 1, 2012·Biomaterials·Guorui JinSeeram Ramakrishna
Nov 2, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brian O DiekmanFarshid Guilak
Mar 12, 2013·The Annals of Otology, Rhinology, and Laryngology·Koshi OtsukiKoichi Omori
May 15, 2013·Respiratory Medicine·Mazen O Al-QadiSidney S Braman
Sep 24, 2013·Experimental Cell Research·Bita CarrionAndrew J Putnam
Aug 1, 2012·Advances in Wound Care·Swathi BalajiTimothy M Crombleholme
Jan 1, 2012·Cells·Atsuyuki InuiA Hari Reddi
Dec 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Su-Hwan KimNathaniel S Hwang
Jun 4, 2016·Journal of Tissue Engineering and Regenerative Medicine·Xing ZhaoMark A Randolph
Jul 15, 2016·The Annals of Otology, Rhinology, and Laryngology·Tendy ChiangChristopher K Breuer
Nov 20, 2016·International Journal of Pediatric Otorhinolaryngology·Jia Xian LawBt Hj Idrus Ruszymah
Jun 13, 2017·The Annals of Otology, Rhinology, and Laryngology·Masakazu IkedaKoichi Omori

❮ Previous
Next ❯

Citations

Oct 13, 2020·Current Opinion in Organ Transplantation·Maria Siemionow
Feb 13, 2021·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Tomonobu EzureKyoichi Matsuzaki
Mar 26, 2021·Tissue Engineering and Regenerative Medicine·Ji Suk ChoiSeong Keun Kwon
Mar 23, 2021·Head & Neck·Harry SpencerAlwyn D'Souza
Apr 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Tarun AgarwalTapas Kumar Maiti
Dec 31, 2020·International Journal of Stem Cells·Myung-Jin ChungKyu-Shik Jeong
May 26, 2021·Journal of Oral Biosciences·Han Ngoc MaiHan-Sung Jung
Jul 3, 2021·Life·Giuseppe DamianoAttilio Ignazio Lo Monte

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
flow cytometry
bioprinting
scanning electron microscopy
PCR
density gradient centrifugation
dissection

Software Mentioned

GraphPad Prism
Image J
Lucion

Related Concepts

Related Feeds

Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.

Related Papers

International Journal of Pediatric Otorhinolaryngology
Jia Xian LawBt Hj Idrus Ruszymah
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Gang LiXiaojun Li
© 2021 Meta ULC. All rights reserved