Transplantation of a fetus with paternal Thy-1(+)CD34(+)cells for chronic granulomatous disease

Bone Marrow Transplantation
M O MuenchM R Harrison

Abstract

A fetus diagnosed with X-linked chronic granulomatous disease was transplanted with Thy-1(+)CD34(+) cells of paternal origin. The transplant was performed at 14 weeks gestation by ultrasound guided injection into the peritoneal cavity. The fetus was delivered at 38 weeks gestation after an otherwise uneventful pregnancy. Umbilical cord blood was collected and used to determine the level of peripheral blood chimerism as well as levels of functional engrafted cells. Flow cytometry was used to detect donor leukocytes identified as HLA-A2(-)B7(+) cells, whereas recipient cells were identified as HLA-A2(+)B7(-) cells. No evidence of donor cell engraftment above a level of 0.01% was found. PCR was used to detect HLA-DRB1*15(+) donor cells among the recipient's HLA-DRB1*15(-) cells, but no engraftment was seen with a sensitivity of 1:1000. The presence of functional, donor-derived neutrophils was assessed by flow cytometry using two different fluorescent dyes that measure reactive oxygen species generated by the phagocyte NADPH oxidase. No evidence of paternal-derived functional neutrophils above a level of 0.15% was observed. Peripheral blood and bone marrow samples were collected at 6 months of age. Neither sample showed engraftment...Continue Reading

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Citations

Aug 9, 2001·Prenatal Diagnosis
Aug 31, 2002·Journal of Hematotherapy & Stem Cell Research·Laurence E ShieldsMagnus Westgren
Oct 9, 2002·Clinical Obstetrics and Gynecology·Alan W Flake
Jan 23, 2016·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Matthew M BoeligWilliam H Peranteau
Jun 16, 2009·Journal of Pediatric Surgery·Meghna V MisraHeung Bae Kim
Nov 24, 2007·Best Practice & Research. Clinical Obstetrics & Gynaecology·Eleonor Tiblad, Magnus Westgren
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Jun 11, 2008·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Demetri MerianosAlan W Flake
May 19, 2001·Blood Cells, Molecules & Diseases·S M OppenheimG B Anderson
Jan 25, 2005·Bone Marrow Transplantation·M O Muench
Mar 17, 2004·Cancer Control : Journal of the Moffitt Cancer Center·Marcus O Muench, Alicia Bárcena

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