Transplantation of acellular dermis and keratinocytes cultured on porous biodegradable microcarriers into full-thickness skin injuries on athymic rats

Burns : Journal of the International Society for Burn Injuries
Håvard SelandGunnar Kratz

Abstract

In search of an optimal transplantation regime for sufficient dermal and epidermal regeneration after a full-thickness skin injury, wounds on athymic rats were grafted with split-thickness skin grafts or acellular human dermis followed by transplantation with human keratinocytes either in single-cell suspension or cultured on porous biodegradable microcarriers. After 2 weeks, all wounds grafted with acellular human dermis showed a well organised and vascularised dermal component and reepithelialisation on the grafted dermal matrix was complete 21 days after transplantation with human keratinocytes. Wounds grafted with human keratinocytes seeded on biodegradable microcarriers or split-thickness skin grafts displayed over time (i.e. 16-21 days post-transplantation) a significantly thicker epithelial cell layer in comparison to wounds grafted with keratinocytes in single-cell suspensions or microcarriers not seeded with cells. Furthermore, measurements of dermal thickness in the closed wounds 21 days after grafting showed a significantly thicker and well organised neodermal component in wounds transplanted with keratinocytes seeded on microcarriers or split-thickness skin grafts compared to all other wounds. Positive immunostainin...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H GreenJ Thomas
Mar 1, 1977·Somatic Cell Genetics·D W LevineW G Thilly
Aug 1, 1992·Pathology, Research and Practice·M WernerA Georgii
Jan 1, 1985·Annals of the New York Academy of Sciences·T T SunR Eichner
Aug 16, 1984·The New England Journal of Medicine·G G GallicoH Green
Jun 1, 1995·Burns : Journal of the International Society for Burn Injuries·D J Wainwright
Jul 1, 1994·The Journal of Burn Care & Rehabilitation·J F HansbroughL Wilkins
Feb 15, 2002·International Journal of Hematology·Cécile V Denis
Apr 17, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mark A Carlson, Michael T Longaker
Oct 12, 2004·Burns : Journal of the International Society for Burn Injuries·Béatrice MisVincent Ronfard
May 1, 2007·Burns : Journal of the International Society for Burn Injuries·Carl-Johan GustafsonGunnar Kratz
May 16, 2009·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Fredrik R M HussGunnar Kratz

❮ Previous
Next ❯

Citations

Aug 15, 2013·Annals of Biomedical Engineering·Waleed O TwalW Scott Argraves
Feb 15, 2014·Advances in Wound Care·Justin R Sharpe, Yella Martin
Jun 5, 2012·Polski przeglad chirurgiczny·Wojciech ŁabuśMariusz Nowak
Dec 29, 2015·Plastic and Reconstructive Surgery·Mansher SinghElof Eriksson
Apr 3, 2016·International Journal of Biological Macromolecules·Mohammad Karimian S AHossein Baniasadi
Nov 22, 2014·Science·Bryan K SunPaul A Khavari
Sep 23, 2016·Journal of Materials Science. Materials in Medicine·Ekaterina I ShishatskayaTatiana G Volova
Nov 30, 2016·International Journal of Molecular Sciences·Atul A ChaudhariShreekumar R Pillai
Aug 28, 2020·Burns : Journal of the International Society for Burn Injuries·Alexander P LarssonJohan P E Junker
Sep 21, 2021·Tissue Engineering. Part C, Methods·Shiva MotamediHassan Niknejad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

© 2021 Meta ULC. All rights reserved