Transplantation of Adipose Tissue-Derived Stem Cells into Brain Through Cerebrospinal Fluid in Rat Models: Protocol Development and Initial Outcome Data

Current Stem Cell Research & Therapy
Naser AminiM Mozafari

Abstract

Cell therapy is an important strategy for the treatment of incurable diseases including those that occur in the Central Nervous System (CNS). Among different strategies, the method of delivering or transplantation of cells into the brain has shown significant effects on regeneration. In this study, a new protocol has been developed for the transplantation of adipose tissuederived stem cells into the brain through Cerebrospinal Fluid (CSF) in rat models. For this purpose, a wide range of ages (7-30 days old) of male neonates of Wistar rats was used. Moreover, human adipose tissue was obtained from a superficial layer of abdomen through liposuction surgery. The size of the inserted part of needle to access middle cranial fossa and subarachnoid space in animals with an average weight of 10-80 g was determined. In addition, to confirm the entrance of needle into the subarachnoid space, CSF was aspirated slowly and then injection was done within two minutes. The findings showed the presence of transplanted human Adipose-Derived Stem Cells (hADSC) in the cerebellum and basal ganglia following three days and also after two months that confirmed the entrance of transplanted cells into the cerebrospinal fluid and migration of them into ...Continue Reading

References

Apr 1, 1995·Neurosurgery·Y Natori, A L Rhoton
Jun 6, 2013·Acta Pharmacologica Sinica·Xiu-ling XuGuang-hui Liu
Jan 29, 2016·Neurotoxicity Research·Naser AminiMohammad Taghi Joghataei
Oct 18, 2016·Journal of Pharmacological and Toxicological Methods·Naser AminiMohammad Taghi Joghataei
Feb 17, 2017·Life Sciences·Sara RamezaniMohammad Taghi Joghataei

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Citations

Jun 5, 2019·Journal of Biomedical Materials Research. Part a·Zohreh ArabpourSeyed N Ostad
Feb 4, 2021·Regenerative Medicine·Mohammed A BananiFarshid Sefat
Dec 8, 2021·Journal of Cellular and Molecular Medicine·Nehad F MazenAmal S El-Shal

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