Abstract
Segmental bone defect is still a challenge to orthopedic surgeons. Currently available therapies for segmental bone defects have some drawbacks. Tissue engineering using pluripotent stem cells is a new, promising method for bone repair. The present study aims to promote the effect of bone defect repair using the tissue engineered bone in combination with vascularized periosteal flaps. The adenoviral vector carrying Cbfa1 transduced rabbit adipose-derived mesenchymal stem cells and gene modified tissue engineering bone (GMB) were constructed. Rabbits with radial defects were implanted with the GMB together with vascularized periosteum (group A); or GMB with free periosteum (group B); or GMB (group C), and scaffold (group D). The bone repair effect was evaluated at 4, 8, or 12 wk, respectively, after the operations. Cbfa1 proteins were strongly expressed in adipose stem cells (ADSCs) that formed a stratified network on the inner surface of the polylactic acid/ polycaprolacton (PLA/PCL) pores. Bone repair was well achieved in the rabbits treated with the Cbfa1-expressing ADSCs and vascularized flap that was markedly better than those treated with either Cbfa1-expressing ADSCs alone or with vascularized flap alone. Combination with...Continue Reading
References
Aug 19, 2000·Endocrine Reviews·A YamaguchiT Suda
Nov 21, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P Ducy
Jul 27, 2001·Mechanisms of Development·E ZelzerB R Olsen
Oct 29, 2003·Current Opinion in Biotechnology·Jeffrey A Hubbell
Nov 5, 2003·Calcified Tissue International·H ZhengG Dang
Oct 12, 2004·Experimental Cell Research·Charles A GersbachAndrés J García
Oct 16, 2004·Biotechnology and Bioengineering·Charles A GersbachAndrés J García
Nov 13, 2004·The Journal of Biological Chemistry·Hiroko Kojima, Toshimasa Uemura
Jan 18, 2005·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M EgermannA W Baltzer
Mar 22, 2005·Gene·Dietmar W Hutmacher, Andres J Garcia
Jun 3, 2005·The Journal of Bone and Joint Surgery. American Volume·E VögelinJ R Lieberman
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xinping ZhangRobert E Guldberg
May 30, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ronald W LindseyLoren L Latta
May 15, 2007·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·V PinsolleJ Baudet
Aug 9, 2007·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Remco de BreeC René Leemans
Dec 8, 2007·Journal of Cellular Physiology·Taro MatsumotoHideo Mugishima
Sep 23, 2008·Injury·Panayotis N SoucacosGeorge Babis
Jan 7, 2010·Annals of Biomedical Engineering·Chunhou AnJianjun Li
Mar 3, 2010·Immunological Reviews·Despina Sitara, Antonios O Aliprantis
Apr 16, 2010·Annals of the New York Academy of Sciences·Brendan F BoyceLianping Xing
Oct 6, 2010·American Journal of Veterinary Research·Chrisoula A ToupadakisClare E Yellowley
Citations
Jul 11, 2012·Journal of Biomedicine & Biotechnology·Wang QingYang Liu
Sep 12, 2013·PloS One·Zhong FangFeng Li
Jun 6, 2013·TheScientificWorldJournal·Sönke Percy FreyRene Zellweger
Mar 12, 2013·Biochemical and Biophysical Research Communications·Peng XueYukun Li
Jul 17, 2012·The Journal of Surgical Research·Ansgar Brüning, Ioannis Mylonas
Jun 22, 2013·Cell Biology International·Lin WangHongchen Liu
Oct 28, 2014·Journal of Orthopaedic Surgery and Research·Shasha ZouHongliang Hu
Dec 10, 2015·BioMed Research International·R V DeevA A Isaev
Dec 7, 2013·BioMed Research International·Marta BarbaWanda Lattanzi
Mar 7, 2019·The Journal of Craniofacial Surgery·Derek B AssersonDavid E Sahar
Jul 9, 2020·Tissue Engineering. Part B, Reviews·Maximilian M MengerTina Histing
Apr 8, 2017·Journal of Veterinary Science·Jaeyong SongByung-Jae Kang
Aug 16, 2019·Tissue Engineering and Regenerative Medicine·Jagadeesh Kumar VenkatesanMagali Cucchiarini
Aug 28, 2014·Cell Biochemistry and Biophysics·Xiaojun XuJianjun Li
Jan 31, 2020·Frontiers in Bioengineering and Biotechnology·David S SparksDietmar W Hutmacher