Transplantation of co-microencapsulated hepatocytes and HUVECs for treatment of fulminant hepatic failure

The International Journal of Artificial Organs
Liyuan QiuChangyong Wang

Abstract

Microencapsulated hepatocytes might solve immunological rejection, broadening a new perspective for the treatment of fulminant hepatic failure (FHF). However, the transplantation of microcapsulated hepatocytes is limited by low cell viability. Nevertheless, the co-microencapsulation of hepatocytes and human umbilical vein endothelial cells (HUVECs) may make the treatment of FHF more promising. We prepared the microcapsules using the high-voltage electrostatic droplet spray method, transplanted the empty microcapsules, isolated hepatocytes, microcapsulated hepatocytes, and co-microencapsulated hepatocytes and HUVEC intraperitoneally into rat models of FHF induced by D-aminogalactose (D-gal). After 1, 3, and 7 days, and 2, 3, and 4 weeks posttransplantation, we calculated the mortality and assessed alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin (ALB) levels in the serum of the model; evaluated the integrality and recovery of microcapsules; and stained with hematoxylin and eosin (H&E) the recovered microcapsules as well as the liver of the FHF rats. Hepatocyte-specific functions, including the levels of ALT, AST, and ALB in the serum of the co-microencapsulation group, were significantly better than ...Continue Reading

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Citations

Jan 25, 2020·Advanced Healthcare Materials·Alain da Silva MoraisRui L Reis
Mar 3, 2015·Biotechnology and Bioengineering·Yufan Xu, Xiaohong Wang
Mar 27, 2015·Journal of Biomedical Materials Research. Part a·Cristina Martínez-RamosManuel Monleón Pradas
Jun 15, 2021·Acta Biomaterialia·Da ZouChun-Xia Zhao

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