PMID: 8614073May 1, 1996Paper

Transplantation of cryopreserved cultured epidermal allografts

The Journal of Trauma
M R MaddenL Staiano-Coico

Abstract

To optimize cryopreservation methods for cultured epidermal allografts (CEAs) for transplantation onto wounds. Conditions were determined to optimize the cryopreservation of CEAs. Cryopreserved CEAs were then grafted onto 16 donor sites in a double blind randomized trial. CEAs were grown in culture. Viability of cryopreserved CEAs was determined by: 1) trypan blue dye exclusion; 2) histology; 3) flow cytometry; and 4) acid lipase activity. Cryopreserved CEAs were grafted onto 16 donor sites. Wound healing assessments included 1) visual assessment of healing; 2) histologic assessment of re-epithelialization and differentiation; and 3) visual scar assessment. CEAs were cryopreserved using a controlled rate freezer with an experimentally determined setting of -7 degrees C for the CEA freezing point. Such freezing conditions resulted in retention of approximately 92% of the original viability, no loss in basal keratinocytes as determined by flow cytometry, and no change in acid lipase activity. CEAs cryopreserved according to this method were grafted onto 16 donor sites in a double blind randomized trial. All donor sites underwent complete healing. Histologic examination of biopsies taken from the center of the wound beds showed th...Continue Reading

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Citations

Apr 12, 2000·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Z Ruszczak, R A Schwartz
Oct 27, 2010·The Journal of Dermatological Treatment·Xinlei HuChunmao Han
Jun 15, 2013·Burns : Journal of the International Society for Burn Injuries·Celine AuxenfansFabienne Braye
Nov 22, 2011·Clinics in Plastic Surgery·Florian GroeberKatja Schenke-Layland
Nov 4, 2011·Burns : Journal of the International Society for Burn Injuries·Zephanie TyackJason Wasiak
Jan 19, 2011·Advanced Drug Delivery Reviews·Florian GroeberKatja Schenke-Layland
Sep 11, 2007·Burns : Journal of the International Society for Burn Injuries·Clarabelle PhamGuy Maddern
Jul 19, 2015·Cirugia y cirujanos·Francisco Martínez-FloresWalter Querevalu-Murillo
Oct 26, 1999·Cryobiology·J PaschG Rau
Nov 16, 2017·Advances in Skin & Wound Care·Jaechul YoonChunjae Yoon
Sep 15, 1999·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·T AokiJ Hayashi

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