Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells

Stem Cell Research & Therapy
Masaki HataKeiko Naruse

Abstract

Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy. DPSCs isolated from the dental pulp of extracted incisors of Sprague-Dawley rats were partly frozen in a -80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation. Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshl...Continue Reading

References

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Citations

Jan 5, 2017·Journal of Tissue Engineering and Regenerative Medicine·Kathleen SanenJames Phillips
Jul 30, 2020·Cell and Tissue Research·Duaa AbuarqoubAbdalla Awidi
Aug 4, 2016·Current Diabetes Reports·Hiroki Mizukami, Soroku Yagihashi
Sep 28, 2018·Stem Cell Research & Therapy·Syed Shadab RazaMohsin Ali Khan
May 18, 2018·Frontiers in Bioengineering and Biotechnology·Mona K Marei, Rania M El Backly
Jan 23, 2021·International Journal of Molecular Sciences·Oana BaruMîndra Badea

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Methods Mentioned

BETA
light microscopy
PCR
electrophoresis

Software Mentioned

CELLQUEST

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