Transplantation of CXCR4 Overexpressed Mesenchymal Stem Cells Augments Regeneration in Degenerated Intervertebral Discs

DNA and Cell Biology
Ji-Nan WeiWen-Hao Tang

Abstract

SDF-1/CXCR4 chemotaxis signals play important roles in regulating the stem cell-based tissue regeneration. The aim of this research is to evaluate whether high expression of CXCR4 enhances the migration of mesenchymal stem cells (MSCs) and increases the efficiency of intervertebral disc (IVD) regeneration. MSCs overexpressing CXCR (CXCR4-MSC) were created by lentiviral-CXCR4-vect transfection, labeled with SPIO, and transplanted into rabbit degenerative IVD induced by annulus puncture. X-ray and T2-weighted MR images of the spine were obtained at 0, 8, and 16 weeks post-transplantation. The transplanted stem cells were traced by both MR imaging and Prussian blue staining. The stem cell-based IVD degeneration was evaluated by quantifying the expression of aggrecan and type II collagen. The in vitro chemotaxis test was performed to study the migration of CXCR4-MSCs to the supplement of SDF-1. The CXCR4-overexpressing MSCs stably elevated the expression of CXCR4 and increased the migration to SDF-1. The SPIO-labeled CXCR4-MSC could be detected within the IVD by MRI till 16 weeks post-transplantation. Prussian blue staining evidenced more SPIO-positive cells within the IVD transplanted with CXCR4-MSCs. Compared to the control group...Continue Reading

References

Apr 1, 2006·Science·Kateri A Moore, Ihor R Lemischka
Dec 18, 2007·The Spine Journal : Official Journal of the North American Spine Society·Satoshi SobajimaJames D Kang
Jan 1, 2008·The Spine Journal : Official Journal of the North American Spine Society·Simon DagenaisScott Haldeman
Dec 30, 2009·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·G W OmlorWiltrud Richter
Dec 1, 2010·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Yun-Tao WangFeng Wang
Jul 28, 2011·Transplantation·Lluis OrozcoJavier García-Sancho
Oct 30, 2013·Nature Reviews. Rheumatology·Makarand V Risbud, Irving M Shapiro
Jan 18, 2014·Connective Tissue Research·Antonio CuestaJosé A Vega
Jun 11, 2014·Nature Reviews. Rheumatology·Yong-Can HuangKeith D K Luk
Jun 21, 2014·Expert Opinion on Therapeutic Targets·Amanda VillalvillaGabriel Herrero-Beaumont
Jul 6, 2014·Advanced Drug Delivery Reviews·Daisuke Sakai, Sibylle Grad
Nov 6, 2014·Development·John Wang, Holger Knaut
Dec 3, 2014·The Spine Journal : Official Journal of the North American Spine Society·Daisuke SakaiJoji Mochida
Jan 27, 2015·World Journal of Stem Cells·Charles HandleyGraham Jenkin
Feb 25, 2015·Nature Reviews. Rheumatology·Daisuke Sakai, Gunnar B J Andersson
Apr 2, 2015·Osteoarthritis and Cartilage·P-P A VergroesenT H Smit

❮ Previous
Next ❯

Citations

Jan 25, 2019·Current Stem Cell Research & Therapy·Marietta Herrmann, Franz Jakob
Jul 15, 2017·Stem Cell Research & Therapy·Amruta Barhanpurkar-NaikMohan R Wani
Jun 15, 2021·Frontiers in Immunology·Huating ChenBinghui Li

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
transgenic
transfection
PCR

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.