Transplantation of expanded bone marrow-derived very small embryonic-like stem cells (VSEL-SCs) improves left ventricular function and remodelling after myocardial infarction.

Journal of Cellular and Molecular Medicine
Ewa K Zuba-SurmaRoberto Bolli

Abstract

Adult bone marrow-derived very small embryonic-like stem cells (VSEL-SCs) exhibit a Sca-1(+)/Lin(-)/CD45(-) phenotype and can differentiate into various cell types, including cardiomyocytes and endothelial cells. We have previously reported that transplantation of a small number (1 × 10(6)) of freshly isolated, non-expanded VSEL-SCs into infarcted mouse hearts resulted in improved left ventricular (LV) function and anatomy. Clinical translation, however, will require large numbers of cells. Because the frequency of VSEL-SCs in the marrow is very low, we examined whether VSEL-SCs can be expanded in culture without loss of therapeutic efficacy. Mice underwent a 30 min. coronary occlusion followed by reperfusion and, 48 hrs later, received an intramyocardial injection of vehicle (group I, n = 11), 1 × 10(5) enhanced green fluorescent protein (EGFP)-labelled expanded untreated VSEL-SCs (group II, n = 7), or 1 × 10(5) EGFP-labelled expanded VSEL-SCs pre-incubated in a cardiogenic medium (group III, n = 8). At 35 days after myocardial infarction (MI), mice treated with pre-incubated VSEL-SCs exhibited better global and regional LV systolic function and less LV hypertrophy compared with vehicle-treated controls. In contrast, transplan...Continue Reading

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Citations

May 3, 2013·Targeted Oncology·Romano Demicheli
Dec 18, 2010·Journal of Cardiovascular Translational Research·Wojciech WojakowskiMichał Tendera
Jan 5, 2011·Antioxidants & Redox Signaling·Ewa K Zuba-SurmaBuddhadeb Dawn
May 24, 2012·Circulation Research·Pearl QuijadaMark A Sussman
May 16, 2013·Journal of Cardiovascular Pharmacology·Jianqin Ye, Yerem Yeghiazarians
Mar 9, 2012·Cardiology Research and Practice·Duc Thang VuTheo Kofidis
Aug 30, 2014·Advances in Medical Sciences·Mariusz Z RatajczakMarcin Moniuszko
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Jun 21, 2012·British Journal of Pharmacology·T JadczykP Madeddu
Sep 21, 2013·Stem Cell Reports·Masanori MiyanishiIrving L Weissman
Sep 11, 2016·Human Reproduction Update·Deepa BhartiyaSreepoorna Unni
Sep 16, 2015·Stem Cells Translational Medicine·Yuri M KlyachkinAhmed Abdel-Latif
Feb 27, 2013·Molecular Reproduction and Development·Susannah H Kassmer, Diane S Krause
May 16, 2014·Journal of Biomaterials Applications·Anna Donesz-SikorskaKrzysztof Marycz

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Methods Mentioned

BETA
flow cytometry
transgenic

Software Mentioned

SPSS
ImageStream
Pro Plus
Prosolv
Image

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