Transplantation of human bone marrow stem cells into symptomatic ALS mice enhances structural and functional blood-spinal cord barrier repair

Experimental Neurology
Svitlana Garbuzova-DavisCesario V Borlongan

Abstract

Accumulating evidence shows alterations in the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) in ALS patients and in animal models of disease, mainly by endothelial cell (EC) damage. Repair of the altered barrier in the CNS by replacement of ECs via cell transplantation may be a new therapeutic approach for ALS. Recently, we demonstrated positive effects towards BSCB repair by intravenous administration of unmodified human bone marrow CD34+ (hBM34+) cells at different doses into symptomatic ALS mice. However, particular benefits of these transplanted cells on microvascular integrity in symptomatic ALS mice are still unclear. The aim of the present study was to determine the structural and functional spinal cord capillary integrity in symptomatic ALS mice after intravenous administration of hBM34+ cells. The G93A mice at 13 weeks of age intravenously received one of three different cell doses (5 × 104, 5 × 105, or 1 × 106) and were euthanized at 17 weeks of age (4 weeks post-transplant). Control groups were media-treated and non-carrier mutant SOD1 gene mice. Capillary ultrastructural (electron microscopy), immunohistochemical (laminin and HuNu), and histological (myelin and capillary density) analyses were perfo...Continue Reading

Citations

Aug 10, 2019·International Journal of Molecular Sciences·Mariángeles KovacsLuis Barbeito
Mar 25, 2020·Cell Transplantation·Svitlana Garbuzova-DavisCesario V Borlongan
Jul 2, 2020·Journal of Cellular and Molecular Medicine·Loree HellerClaude Nicolau
Dec 10, 2020·Journal of Neurotrauma·Lin-Yu JinHai-Ying Liu
Jun 5, 2021·Brain Circulation·Nadia SadanandanSvitlana Garbuzova-Davis

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