Transplantation of human telomerase reverse transcriptase gene-transfected Schwann cells for repairing spinal cord injury

Neural Regeneration Research
Shu-Quan ZhangRui Gu

Abstract

Transfection of the human telomerase reverse transcriptase (hTERT) gene has been shown to increase cell proliferation and enhance tissue repair. In the present study, hTERT was transfected into rat Schwann cells. A rat model of acute spinal cord injury was established by the modified free-falling method. Retrovirus PLXSN was injected at the site of spinal cord injury as a vector to mediate hTERT gene-transfected Schwann cells (1 × 10(10)/L; 10 μL) or Schwann cells (1 × 10(10)/L; 10 μL) without hTERT gene transfection. Between 1 and 4 weeks after model establishment, motor function of the lower limb improved in the hTERT-transfected group compared with the group with non-transfected Schwann cells. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and reverse transcription-polymerase chain reaction results revealed that the number of apoptotic cells, and gene expression of aquaporin 4/9 and matrix metalloproteinase 9/2 decreased at the site of injury in both groups; however, the effect improved in the hTERT-transfected group compared with the Schwann cells without hTERT transfection group. Hematoxylin and eosin staining, PKH26 fluorescent labeling, and electrophysiological testing demonstrated that compared wi...Continue Reading

Citations

Jun 21, 2017·Stem Cells International·Jing Qu, Huanxiang Zhang
May 5, 2017·Neural Regeneration Research·Wei RongCheng Zhang

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Methods Mentioned

BETA
transfect
flow cytometry
transfection
PCR
electrophoresis
fluorescence microscopy

Software Mentioned

SPSS
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