Transplantation of induced pluripotent stem cell-derived neurospheres for peripheral nerve repair

Biochemical and Biophysical Research Communications
Takuya UemuraHiroaki Nakamura

Abstract

In spite of the extensive research using induced pluripotent stem (iPS) cells, the therapeutic potential of iPS cells in the treatment of peripheral nerve injury is largely unknown. In this study, we repaired peripheral nerve gaps in mice using tissue-engineered bioabsorbable nerve conduits coated with iPS cell-derived neurospheres. The secondary neurospheres derived from mouse iPS cells were suspended in each conduit (4000,000 cells per conduit) and cultured in the conduit in three-dimensional (3D) culture for 14 days. We then implanted them in the mouse sciatic nerve gaps (5 mm) (iPS group; n=10). The nerve conduit alone was implanted in the control group (n=10). After 4, 8 and 12 weeks, motor and sensory functional recovery in mice were significantly better in the iPS group. At 12 weeks, all the nerve conduits remained structurally stable without any collapse and histological analysis indicated axonal regeneration in the nerve conduits of both groups. However, the iPS group showed significantly more vigorous axonal regeneration. The bioabsorbable nerve conduits created by 3D-culture of iPS cell-derived neurospheres promoted regeneration of peripheral nerves and functional recovery in vivo. The combination of iPS cell technol...Continue Reading

References

Feb 13, 2003·Journal of Reconstructive Microsurgery·Laura T GeorgeSusan E Mackinnon
Feb 21, 2006·Experimental Neurology·Fátima Rosalina Pereira LopesAna Maria Blanco Martinez
May 19, 2006·Biochemical and Biophysical Research Communications·Hirofumi SuemoriNorio Nakatsuji
Mar 29, 2008·Cell Stem Cell·Marius WernigRudolf Jaenisch
Jan 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dan XuYupo Ma
Jul 11, 2009·Nature Biotechnology·Kyoko MiuraShinya Yamanaka
Jan 27, 2010·Journal of Materials Science. Materials in Medicine·Lei Zheng, Hui-Fei Cui
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Osahiko TsujiHideyuki Okano
Aug 21, 2010·Journal of Biomedical Materials Research. Part a·Kai-Hei TsePaul J Kingham
Apr 26, 2011·Biomaterials·Aijun WangSong Li
Jun 21, 2011·International Journal of Molecular Medicine·Guibo LiuXiaojie Tong
Jul 6, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Keisuke Okita, Shinya Yamanaka
Sep 13, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Osahiko TsujiHideyuki Okano
Sep 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Satoshi NoriHideyuki Okano

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Citations

Sep 17, 2013·Cell Biochemistry and Biophysics·Ruijun LiLaijin Lu
Jun 4, 2014·Journal of Tissue Engineering and Regenerative Medicine·Yorikatsu WatanabeTeruo Okano
Dec 23, 2015·Neural Regeneration Research·Rafet ÖzayYusuf Şükrü Çağlar
May 14, 2014·Biomaterials·Xiaosong GuDavid F Williams
Apr 12, 2015·Interactive Cardiovascular and Thoracic Surgery·Nobuyoshi TanakaHirofumi Takemura
Dec 24, 2014·Cytotherapy·Cuiping ZhangXiaobing Fu
Sep 9, 2016·Cell and Tissue Banking·Martin BoháčJán Koller
Jun 4, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mattias LeinoVéronique Chotteau
Dec 2, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hanako NishimotoYoshitada Sakai
Sep 10, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Takuya YokoiHiroaki Nakamura
Oct 21, 2017·Journal of Neurosurgery·Kosuke ShintaniHiroaki Nakamura
Aug 18, 2018·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mari ShimizuHiroyuki Sakurai
Oct 27, 2018·Cell Biology International·Khairunnisa RamliMin Hwei Ng
May 9, 2014·The International Journal of Artificial Organs·Bill G X ZhangPeter F M Choong
Jul 19, 2016·Reviews in the Neurosciences·Qi QuanQing Zhao
Dec 20, 2012·Annals of Plastic Surgery·Taolin FangFeng Zhang
Aug 19, 2020·International Journal of Molecular Sciences·Soja Saghar Soman, Sanjairaj Vijayavenkataraman
Nov 16, 2018·International Journal of Molecular Medicine·Vesna BucanChristine Radtke
Jul 10, 2020·Expert Opinion on Biological Therapy·Tetsuro Tamaki
Jun 20, 2021·The International Journal of Artificial Organs·Ryosuke TamuraHiroto Terashi
Jul 10, 2017·ACS Biomaterials Science & Engineering·William A LackingtonFergal J O'Brien
Aug 28, 2021·Biology·Philip Jalink, Massimiliano Caiazzo

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