Transplanted clonal neural stem-like cells respond to remote photic stimulation following incorporation within the suprachiasmatic nucleus

Experimental Neurology
Piotr ZlomanczukWilliam J Schwartz

Abstract

Multipotent neural stem-like cells (NSCs) obtained from one brain region and transplanted to another region appear to differentiate into neuronal and glial phenotypes indigenous to the implantation site. Whether these donor-derived cells are appropriately integrated remains unanswered. In order to test this possibility, we exploited the suprachiasmatic nucleus (SCN) of the hypothalamus, site of a known circadian clock, as a novel engraftment target. When a clone of NSCs initially derived from neonatal mouse cerebellum was transplanted into mouse embryos, the cells incorporated within the SCN over a narrow gestational window that corresponded to the conclusion of SCN neurogenesis. Immunocytochemical staining suggested that donor-derived cells in the SCN synthesized a peptide neurotransmitter (arginine vasopressin) characteristic of SCN neurons. Donor-derived SCN cells reacted to light pulses by expressing immunoreactive c-Fos protein in a pattern that is appropriate for native SCN cells. This region-specific and physiologically appropriate response to the natural stimulation of a remote sensory input implies that donor-derived and endogenous cells formed true SCN chimeras, suggesting that exogenous NSCs engrafted to ectopic loca...Continue Reading

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Citations

Aug 6, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jaime ImitolaEvan Y Snyder
Dec 31, 2003·Molecular and Cellular Neurosciences·John A BoockvarDonald M O'Rourke
May 16, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Stephen YipEvan Y Snyder
Jul 5, 2005·Experimental Neurology·Mark A ParkerEvan Y Snyder
Mar 30, 2004·Journal of Neuroscience Research·Evan Y SnyderMargaret Goodell
May 29, 2002·Gene Therapy·K I ParkE Y Snyder
May 19, 2009·Expert Review of Anticancer Therapy·Thomas KosztowskiAlfredo Quiñones-Hinojosa

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