Transplanted neural-like cells improve memory and Alzheimer-like pathology in a rat model

Cytotherapy
Elham HoveiziAzade Eskandary

Abstract

Degeneration of the central nerve system, particularly in Alzheimer's disease, is a burden on society, and despite years of research, there is no effective treatment. Cell therapy appears to be an option that is of growing interest in neural studies. The main aim of this study was to investigate the histological and physiological effects of transplantation the neuron-like cell (NLC)-derived mouse embryonic stem cells (mESCs) on the repair of brain lesions in an Alzheimer's animal model (AM) in rats. Behavioral experiments were conducted in the light hours in a Y-shaped maze device. Animals were randomly divided into five groups, with seven rats per group. The nucleus basalis of Meynert (NBM) was destroyed bilaterally with an electrical lesion (0.5 mA for 3 s). One week after the bilateral lesion of the NBM, the differentiated NLCs (0.1 mL) were injected with stereotaxic surgery using a Hamilton syringe at NBM coordinates, and behavioral and histological tests were performed by the Y-maze task and hematoxylin and eosin staining after five weeks of the lesion. Also, differentiated cells detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis and fluorescent immunostaining. The expression of neu...Continue Reading

Citations

Feb 9, 2019·Journal, Genetic Engineering & Biotechnology·Dalia FleifelSherif F El-Khamisy
Dec 20, 2018·Molecular Biology Reports·Masoume AlipourKoorosh Shahpasand
Mar 3, 2021·European Journal of Medicinal Chemistry·Sukriti SrivastavaSunil Kumar Khare
Jun 27, 2021·Bulletin of Experimental Biology and Medicine·V Kh KhavinsonR S Umnov

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