Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance.

Transgenic Research
Wendy CraigTeodoro Cardi

Abstract

The possibility of altering the unsaturation level of fatty acids in plant lipids by genetic transformation has implications for the stress tolerance of higher plants as well as for their nutritional value and industrial utilisation. While the integration and expression of transgenes in the plastome has several potential advantages over nuclear transformation, very few attempts have been made to manipulate fatty acid biosynthesis using plastid transformation. We produced transplastomic tobacco plants that express a Delta(9) desaturase gene from either the wild potato species Solanum commersonii or the cyanobacterium Anacystis nidulans, using PEG-mediated DNA uptake by protoplasts. Incorporation of chloroplast antibiotic-insensitive point mutations in the transforming DNA was used to select transformants. The presence of the transcript and the Delta(9) desaturase protein in transplastomic plants was confirmed by northern and western blot analyses. In comparison with control plants, transplastomic plants showed altered fatty acid profiles and an increase in their unsaturation level both in leaves and seeds. The two transgenes produced comparable results. The results obtained demonstrate the feasibility of using plastid transforma...Continue Reading

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Citations

Jul 14, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Brigitta DudasPal Maliga
Mar 8, 2012·Protoplasma·Jelli Venkatesh, Se Won Park
Mar 26, 2011·Plant Molecular Biology·Jihong Liu Clarke, Henry Daniell
Mar 19, 2013·Plant Molecular Biology·Ralph Bock
Jan 18, 2011·Plant Physiology·Pal Maliga, Ralph Bock
Jun 14, 2008·Transgenic Research·Francesca De MarchisMichele Bellucci
Aug 17, 2011·Biotechnology Advances·Nunzia ScottiTeodoro Cardi
Oct 6, 2009·Biochemical and Biophysical Research Communications·Jian-Tao ZhangHong-Xia Zhang
May 4, 2011·Plant Biotechnology Journal·Marcelo Rogalski, Helaine Carrer
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Aug 16, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beibei XiangYong Wang
Mar 2, 2017·Cold Spring Harbor Perspectives in Biology·Christian R BoehmJim Haseloff
May 20, 2020·Scientific Reports·Ryan W ChristianAmit Dhingra
Jun 17, 2018·Plant Science : an International Journal of Experimental Plant Biology·Agnieszka A PiatekC Neal Stewart
Apr 27, 2021·Plant Communications·Shengchun LiJiang Zhang

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